%0 Journal Article %1 Xie2007 %A Xie, Yuqun %A He, Jin %A Huang, Jun %A Zhang, Jibin %A Yu, Ziniu %D 2007 %J Journal of Agricultural and Food Chemistry %K %N 17 %P 6823-6828 %R 10.1021/jf070125o %T Determination of 2-Methylisoborneol and Geosmin Produced by Streptomyces sp. and Anabaena PCC7120 %U http://pubs.acs.org/doi/abs/10.1021/jf070125o %V 55 %X A new sample preparation and enrichment technique, headspace liquid-phase microextraction (HS-LPME) linked to gas chromatography−mass spectrometry (GC-MS), was developed for the determination of the off-flavor odorants, 2-methylisoborneol and geosmin, produced by Streptomyces sp. and Anabaena PCC7120. Some of the factors that influence the extraction efficiency of HS-LPME, such as the type of extraction solvent, ionic strength of sample solution, and sample agitation rate, were studied and optimized by a single factor test. Other factors, including extraction temperature, extraction time, microdrop volume, and headspace volume were optimized by orthogonal array design. Extraction of 2-methylisoborneol and geosmin was conducted by exposing 2.5 μL of 1-hexanol for 9 min at 50 °C in the headspace of a 20 mL vial with a 10 mL of sample solution saturated by NaCl and stirred at 800 rpm. The developed protocol demonstrated good repeatability (relative standard deviations (RSDs) < 5%), wide linear ranges (10−5000 ng/L, r2 > 0.999), and low limits of detection (LODs) for 2-methylisoborneol and geosmin (0.05 ng/L for both analytes). Subsequently, the method was successfully applied to extract the analytes in bacterial cultures with high recoveries (from 94% to 98%). Compared with headspace solid-phase microextraction (HS-SPME), HS-LPME demonstrates better linearity, precision, and recovery. Importantly, the sensitivity is about 1 order of magnitude higher than that of most HS-SPME. The results showed that HS-LPME coupled with GC-MS is a simple, convenient, rapid, sensitive, and effective method for the qualitative and quantitative analysis of 2-methylisoborneol and geosmin. Keywords: 2-Methylisoborneol; geosmin; gas chromatography−mass spectrometry (GC-MS); headspace liquid-phase microextraction (HS-LPME); Streptomyces sp.; Anabaena PCC7120

@article{Xie2007, abstract = { A new sample preparation and enrichment technique, headspace liquid-phase microextraction (HS-LPME) linked to gas chromatography−mass spectrometry (GC-MS), was developed for the determination of the off-flavor odorants, 2-methylisoborneol and geosmin, produced by Streptomyces sp. and Anabaena PCC7120. Some of the factors that influence the extraction efficiency of HS-LPME, such as the type of extraction solvent, ionic strength of sample solution, and sample agitation rate, were studied and optimized by a single factor test. Other factors, including extraction temperature, extraction time, microdrop volume, and headspace volume were optimized by orthogonal array design. Extraction of 2-methylisoborneol and geosmin was conducted by exposing 2.5 μL of 1-hexanol for 9 min at 50 °C in the headspace of a 20 mL vial with a 10 mL of sample solution saturated by NaCl and stirred at 800 rpm. The developed protocol demonstrated good repeatability (relative standard deviations (RSDs) < 5%), wide linear ranges (10−5000 ng/L, r2 > 0.999), and low limits of detection (LODs) for 2-methylisoborneol and geosmin (0.05 ng/L for both analytes). Subsequently, the method was successfully applied to extract the analytes in bacterial cultures with high recoveries (from 94% to 98%). Compared with headspace solid-phase microextraction (HS-SPME), HS-LPME demonstrates better linearity, precision, and recovery. Importantly, the sensitivity is about 1 order of magnitude higher than that of most HS-SPME. The results showed that HS-LPME coupled with GC-MS is a simple, convenient, rapid, sensitive, and effective method for the qualitative and quantitative analysis of 2-methylisoborneol and geosmin. Keywords: 2-Methylisoborneol; geosmin; gas chromatography−mass spectrometry (GC-MS); headspace liquid-phase microextraction (HS-LPME); Streptomyces sp.; Anabaena PCC7120 }, added-at = {2011-09-30T23:07:29.000+0200}, author = {Xie, Yuqun and He, Jin and Huang, Jun and Zhang, Jibin and Yu, Ziniu}, biburl = {https://www.bibsonomy.org/bibtex/28923d0a6eaeaf1251477fc01d9d895ef/afcallender}, doi = {10.1021/jf070125o}, eprint = {http://pubs.acs.org/doi/pdf/10.1021/jf070125o}, groups = {public}, interhash = {92171019726e8533de39dee8ea051d89}, intrahash = {8923d0a6eaeaf1251477fc01d9d895ef}, journal = {Journal of Agricultural and Food Chemistry}, keywords = {}, number = 17, pages = {6823-6828}, timestamp = {2011-09-30T23:07:29.000+0200}, title = {Determination of 2-Methylisoborneol and Geosmin Produced by Streptomyces sp. and Anabaena PCC7120}, url = {http://pubs.acs.org/doi/abs/10.1021/jf070125o}, username = {afcallender}, volume = 55, year = 2007 }