%0 Journal Article %1 fluit_evaluation_2010 %A Fluit, A C %A Terlingen, A M %A Andriessen, L %A Ikawaty, R %A van Mansfeld, R %A Top, J %A Stuart, J W Cohen %A van Hall, M A Leverstein %A Boel, C H E %D 2010 %J Journal of Clinical Microbiology %K imported %R 10.1128/JCM.01191-10 %T Evaluation of the DiversiLab system for the detection of hospital outbreaks of different bacterial species %U http://www.ncbi.nlm.nih.gov/pubmed/20861340 %X Many bacterial typing methods are either specific for one species only, time-consuming or poorly reproducible. DiversiLab (DL) (BioMérieux) potentially overcomes these limitations. In this study we evaluated the DL system for the identification of hospital outbreaks for a number bacterial species. Appropriately typed clinical isolates were tested with DL. DL typing agreed with Pulsed-Field Gel-Electrophoresis (PFGE) for Acinetobacter (n=26) and Stenotrophomonas maltophilia (n=13). With 2 exceptions DL typing of Klebsiella (n=23) also correlated with PFGE and in addition PFGE-nontypable isolates (PFGE-NT) could be typed. Enterobacter (n=28) results also correlated with PFGE, also PFGE-NTs could be clustered. In a larger study (n=270) a cluster of 30 isolates was observed that could be subdivided by PFGE. The results for Escherichia coli (n=38) correlated less well with an experimental MultiLocus Variable Number of Tandem Repeat Analysis (MLVA) scheme. Pseudomonas aeruginosa (N=52) showed only a limited number of amplification products for most isolates. When multiple Pseudomonas isolates were assigned to a single type in DL all except one showed multiple MultiLocus Sequence Types. MRSA generally also showed a limited number of amplification products. Isolates that belonged to different outbreaks by other typing methods including PFGE, spa-typing and MLVA were in a number of cases grouped together. For Enterococcus faecium the limited variability in amplification products made interpretation difficult and correlation with MLVA and esp gene typing was poor. All results are reflected in the Simpson's Index of Diversity, Rand's Adjusted and Wallace's Coefficients. DL is a useful tool to help identify hospital outbreaks of Acinetobacter spp., S. maltophilia, the E. cloacae complex, Klebsiella spp. and to a somewhat lesser extend E. coli. In our study DL was inadequate for P. aeruginosa, E. faecium and MRSA. However, it should be noted that for the identification of outbreaks epidemiological data should be combined with typing results.

@article{fluit_evaluation_2010, abstract = {Many bacterial typing methods are either specific for one species only, time-consuming or poorly reproducible. {DiversiLab} {(DL)} {(BioMérieux)} potentially overcomes these limitations. In this study we evaluated the {DL} system for the identification of hospital outbreaks for a number bacterial species. Appropriately typed clinical isolates were tested with {DL.} {DL} typing agreed with {Pulsed-Field} {Gel-Electrophoresis} {(PFGE)} for Acinetobacter (n=26) and Stenotrophomonas maltophilia (n=13). With 2 exceptions {DL} typing of Klebsiella (n=23) also correlated with {PFGE} and in addition {PFGE-nontypable} isolates {(PFGE-NT)} could be typed. Enterobacter (n=28) results also correlated with {PFGE,} also {PFGE-NTs} could be clustered. In a larger study (n=270) a cluster of 30 isolates was observed that could be subdivided by {PFGE.} The results for Escherichia coli (n=38) correlated less well with an experimental {MultiLocus} Variable Number of Tandem Repeat Analysis {(MLVA)} scheme. Pseudomonas aeruginosa {(N=52)} showed only a limited number of amplification products for most isolates. When multiple Pseudomonas isolates were assigned to a single type in {DL} all except one showed multiple {MultiLocus} Sequence Types. {MRSA} generally also showed a limited number of amplification products. Isolates that belonged to different outbreaks by other typing methods including {PFGE,} spa-typing and {MLVA} were in a number of cases grouped together. For Enterococcus faecium the limited variability in amplification products made interpretation difficult and correlation with {MLVA} and esp gene typing was poor. All results are reflected in the Simpson's Index of Diversity, Rand's Adjusted and Wallace's Coefficients. {DL} is a useful tool to help identify hospital outbreaks of Acinetobacter spp., S. maltophilia, the E. cloacae complex, Klebsiella spp. and to a somewhat lesser extend E. coli. In our study {DL} was inadequate for P. aeruginosa, E. faecium and {MRSA.} However, it should be noted that for the identification of outbreaks epidemiological data should be combined with typing results.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Fluit, A C and Terlingen, A M and Andriessen, L and Ikawaty, R and van Mansfeld, R and Top, J and Stuart, J W Cohen and van Hall, M A Leverstein and Boel, C H E}, biburl = {https://www.bibsonomy.org/bibtex/28a791502293b861cfe538968d7d5aa97/jelias}, doi = {10.1128/JCM.01191-10}, interhash = {9d4f7b21bdf7b64ea91bc9e0cc819066}, intrahash = {8a791502293b861cfe538968d7d5aa97}, issn = {{1098-660X}}, journal = {Journal of Clinical Microbiology}, keywords = {imported}, month = sep, note = {{PMID:} 20861340}, timestamp = {2011-03-11T10:06:38.000+0100}, title = {Evaluation of the {DiversiLab} system for the detection of hospital outbreaks of different bacterial species}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20861340}, year = 2010 }