%0 Journal Article %1 citeulike:488464 %A Rabbany, S. Y. %A Kusterbeck, A. W. %A Bredehorst, R. %A Ligler, F. S. %C Department of Engineering, Hofstra University, Hempstead, NY 11550. %D 1994 %J J Immunol Methods %K displacement methodology ag_detection statistic %N 2 %P 227--234 %R 10.1016/0022-1759(94)90059-0 %T Effect of antibody density on the displacement kinetics of a flow immunoassay. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8308297 %V 168 %X This study investigates the effect of antibody density on the kinetics of a solid-phase displacement immunoassay. Conducted in flow under nonequilibrium conditions, the assay utilizes a monoclonal antibody to the cocaine metabolite benzoylecgonine, which has been immobilized onto Sepharose beads and saturated with fluorophore labeled antigen. Displacement of antibody-bound labeled antigen by non-labeled antigen occurs when sample is introduced in the buffer flow. Comparison of matrices coated with two different antibody densities revealed that the displacement efficiency is a function of the density of antibody-bound labeled antigen. A higher density of antibody provides a higher amount of displaced labeled antigen, but the displacement efficiency of the assay is decreased. The effect of antibody density on the immunoassay kinetics was analyzed using a mathematical formulation developed to characterize antibody-antigen interactions at solid-liquid interfaces. Higher antibody density proved to be associated with a lower apparent dissociation rate constant. The implications of these results on the design of immunoassays in flow are discussed.

@article{citeulike:488464, abstract = {This study investigates the effect of antibody density on the kinetics of a solid-phase displacement immunoassay. Conducted in flow under nonequilibrium conditions, the assay utilizes a monoclonal antibody to the cocaine metabolite benzoylecgonine, which has been immobilized onto Sepharose beads and saturated with fluorophore labeled antigen. Displacement of antibody-bound labeled antigen by non-labeled antigen occurs when sample is introduced in the buffer flow. Comparison of matrices coated with two different antibody densities revealed that the displacement efficiency is a function of the density of antibody-bound labeled antigen. A higher density of antibody provides a higher amount of displaced labeled antigen, but the displacement efficiency of the assay is decreased. The effect of antibody density on the immunoassay kinetics was analyzed using a mathematical formulation developed to characterize antibody-antigen interactions at solid-liquid interfaces. Higher antibody density proved to be associated with a lower apparent dissociation rate constant. The implications of these results on the design of immunoassays in flow are discussed.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Engineering, Hofstra University, Hempstead, NY 11550.}, author = {Rabbany, S. Y. and Kusterbeck, A. W. and Bredehorst, R. and Ligler, F. S.}, biburl = {https://www.bibsonomy.org/bibtex/2fe6ee91c9cf344ac0ed56047180650f0/biblio24}, citeulike-article-id = {488464}, doi = {10.1016/0022-1759(94)90059-0}, interhash = {a7cfacd479e79d35cfe4a6d0185f36d5}, intrahash = {fe6ee91c9cf344ac0ed56047180650f0}, issn = {0022-1759}, journal = {J Immunol Methods}, keywords = {displacement methodology ag_detection statistic}, month = {February}, number = 2, pages = {227--234}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Effect of antibody density on the displacement kinetics of a flow immunoassay.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=8308297}, volume = 168, year = 1994 }