%0 Journal Article %1 Bunemann1993 %A Bunemann, M. %A Pott, L. %D 1993 %J Pflugers Arch %K Acetylcholine/metabolism Adenosine Albumin/metabolism Albumins/*metabolism Animals Cell Channels/*metabolism Cultured GTP-Binding Guanosine Guinea Humans Membrane/drug Muscarinic/*metabolism Myocardium/cytology/*metabolism Pigs Potassium Proteins/metabolism Serum Triphosphate/metabolism Triphosphate/pharmacology effects/metabolism Receptor %N 3-4 %P 329-34 %T Membrane-delimited activation of muscarinic K current by an albumin-associated factor in guinea-pig atrial myocytes %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8309794 %V 425 %X Atrial myocytes obtained by enzymatic perfusion of hearts from adult guinea-pigs and cultured for 0-14 days were studied using different configurations of the patch-clamp technique. Activation of muscarinic K current IK(ACh) in whole-cell voltage-clamp mode by strongly diluted sera from various sources could be mimicked by corresponding concentrations of albumin, but not by delipidated ("fatty-acid-free") samples of albumin. In cell-attached membrane patches activity of IK(ACh) channels was significantly higher than basal IK(ACh) channel activity, if the pipette contained serum, whereas application of serum-containing solution to the cell outside the patch did not affect channel activity. In isolated inside-out membrane patches, strong IK(ACh) activation by internal guanosine triphosphate (GTP, 5 microM) was observed if the pipette contained serum. If no activator was presented to the outer face of the membrane, only weak opening activity was observed during bath application of GTP. These results demonstrate that the serum factor which causes activation of IK(ACh) is associated with albumin. Furthermore activation of IK(ACh) by that factor proceeds analogous to ACh or adenosine, i.e. via a membrane-delimited receptor, G-protein, channel interaction.

@article{Bunemann1993, abstract = {Atrial myocytes obtained by enzymatic perfusion of hearts from adult guinea-pigs and cultured for 0-14 days were studied using different configurations of the patch-clamp technique. Activation of muscarinic K current [IK(ACh)] in whole-cell voltage-clamp mode by strongly diluted sera from various sources could be mimicked by corresponding concentrations of albumin, but not by delipidated ("fatty-acid-free") samples of albumin. In cell-attached membrane patches activity of IK(ACh) channels was significantly higher than basal IK(ACh) channel activity, if the pipette contained serum, whereas application of serum-containing solution to the cell outside the patch did not affect channel activity. In isolated inside-out membrane patches, strong IK(ACh) activation by internal guanosine triphosphate (GTP, 5 microM) was observed if the pipette contained serum. If no activator was presented to the outer face of the membrane, only weak opening activity was observed during bath application of GTP. These results demonstrate that the serum factor which causes activation of IK(ACh) is associated with albumin. Furthermore activation of IK(ACh) by that factor proceeds analogous to ACh or adenosine, i.e. via a membrane-delimited receptor, G-protein, channel interaction.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Bunemann, M. and Pott, L.}, biburl = {https://www.bibsonomy.org/bibtex/2f3bf661bc89811c2aa24256d9a9e897b/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {ae5213d75a5cc2dccd84ef4b01761b58}, intrahash = {f3bf661bc89811c2aa24256d9a9e897b}, issn = {0031-6768 (Print) 0031-6768 (Linking)}, journal = {Pflugers Arch}, keywords = {Acetylcholine/metabolism Adenosine Albumin/metabolism Albumins/*metabolism Animals Cell Channels/*metabolism Cultured GTP-Binding Guanosine Guinea Humans Membrane/drug Muscarinic/*metabolism Myocardium/cytology/*metabolism Pigs Potassium Proteins/metabolism Serum Triphosphate/metabolism Triphosphate/pharmacology effects/metabolism Receptor}, month = Nov, note = {Bunemann, M Pott, L Research Support, Non-U.S. Gov't Germany Pflugers Archiv : European journal of physiology Pflugers Arch. 1993 Nov;425(3-4):329-34.}, number = {3-4}, pages = {329-34}, shorttitle = {Membrane-delimited activation of muscarinic K current by an albumin-associated factor in guinea-pig atrial myocytes}, timestamp = {2010-12-14T18:20:48.000+0100}, title = {Membrane-delimited activation of muscarinic K current by an albumin-associated factor in guinea-pig atrial myocytes}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8309794}, volume = 425, year = 1993 }