%0 Journal Article %1 Banks.1998 %A Banks, R. E. %A Forbes, M. A. %A Searles, J. %A Pappin, D. %A Canas, B. %A Rahman, D. %A Kaufmann, S. %A Walters, C. E. %A Jackson, A. %A Eves, P. %A Linton, G. %A Keen, J. %A Walker, J. J. %A Selby, P. J. %D 1998 %J Mol Hum.Reprod. %K & A Acid Amino Binding Blood Cells Chain Chemistry Data Endothelial Factor Factors Female Fetal Growth Human Humans Kinase Kinases Lymphokines Male Molecular Placenta Polymerase Pregnancy Protein Protein-Tyrosine Proteins Proto-Oncogene Reaction Receptor Receptor-1 Research Reverse Sequence Solubility Transcriptase Umbilical Vascular Veins Weight blood cells cytology identify isolation metabolism protein purification %N 4 %P 377-386 %T Evidence for the existence of a novel pregnancy-associated soluble variant of the vascular endothelial growth factor receptor, Flt-1 %U PM:9620838 %V 4 %X Angiogenesis is essential in physiological processes including ovulation, implantation and pregnancy. One of the most potent regulators is the cytokine vascular endothelial growth factor (VEGF). We provide evidence for a novel pregnancy-associated soluble variant of the VEGF receptor Flt-1. VEGF ranged from undetectable to 157.3 pg/ml (mean 49.9 pg/ml, SD 48.4 pg/ml) in plasma samples from normal volunteers (n = 10), but was undetectable in plasma from pregnant women (n = 12) and amniotic fluid (n = 10). Recoveries of spiked VEGF were poor in pregnancy-related samples, indicating the presence of VEGF-binding activity which was confirmed using biosensor and chromatographic techniques. Partial purification and protein sequencing indicated a novel soluble form of Flt-1 with a subunit size of 150 kDa. Normally present as a multimeric structure of approximately 400-550 kDa, complexes of 600-700 kDa were formed following binding of multiple VEGF molecules. Reverse transcriptase polymerase ch

@article{Banks.1998, abstract = {Angiogenesis is essential in physiological processes including ovulation, implantation and pregnancy. One of the most potent regulators is the cytokine vascular endothelial growth factor (VEGF). We provide evidence for a novel pregnancy-associated soluble variant of the VEGF receptor Flt-1. VEGF ranged from undetectable to 157.3 pg/ml (mean 49.9 pg/ml, SD 48.4 pg/ml) in plasma samples from normal volunteers (n = 10), but was undetectable in plasma from pregnant women (n = 12) and amniotic fluid (n = 10). Recoveries of spiked VEGF were poor in pregnancy-related samples, indicating the presence of VEGF-binding activity which was confirmed using biosensor and chromatographic techniques. Partial purification and protein sequencing indicated a novel soluble form of Flt-1 with a subunit size of 150 kDa. Normally present as a multimeric structure of approximately 400-550 kDa, complexes of 600-700 kDa were formed following binding of multiple VEGF molecules. Reverse transcriptase polymerase ch}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Banks, R. E. and Forbes, M. A. and Searles, J. and Pappin, D. and Canas, B. and Rahman, D. and Kaufmann, S. and Walters, C. E. and Jackson, A. and Eves, P. and Linton, G. and Keen, J. and Walker, J. J. and Selby, P. J.}, biburl = {https://www.bibsonomy.org/bibtex/2ce630463e6968e4ba136f8c0e953ca07/kanefendt}, interhash = {bba73e226aa02fa0a7246e2b7b775603}, intrahash = {ce630463e6968e4ba136f8c0e953ca07}, journal = {Mol Hum.Reprod.}, keywords = {& A Acid Amino Binding Blood Cells Chain Chemistry Data Endothelial Factor Factors Female Fetal Growth Human Humans Kinase Kinases Lymphokines Male Molecular Placenta Polymerase Pregnancy Protein Protein-Tyrosine Proteins Proto-Oncogene Reaction Receptor Receptor-1 Research Reverse Sequence Solubility Transcriptase Umbilical Vascular Veins Weight blood cells cytology identify isolation metabolism protein purification}, number = 4, pages = {377-386}, timestamp = {2010-02-05T11:28:47.000+0100}, title = {Evidence for the existence of a novel pregnancy-associated soluble variant of the vascular endothelial growth factor receptor, Flt-1}, url = {PM:9620838}, volume = 4, year = 1998 }