%0 Journal Article %1 citeulike:613250 %A Ius, A. %A Bacigalupo, M. A. %A Longhi, R. %A Meroni, G. %C Istituto di Biocatalisi e Riconoscimento Molecolare, C.N.R., Milan, Italy. iusWico.mi.cnr.it %D 2000 %J Fresenius J Anal Chem %K lytic_peptide immunoassay liposome mellitin %N 8 %P 869--872 %T Selectively conjugated melittins for liposome time-resolved fluoroimmunoassay of theophylline in serum. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11227424 %V 366 %X Theophylline (Th) has been selectively conjugated to the four amino groups of melittin (Mel) by solid phase peptide synthesis. The cytolytic activity of the resultant Th-Mel compounds was tested on liposomes trapping the bovine serum albumin (BSA) conjugate with 4,7-bis(chlorosulfophenyl)-1,10-phenanthrol ine-2,9-dicarboxylic acid (BCPDA). The loss of lytic activity was the highest for Th-K7-Mel. Th-G1-Mel retains almost the same lytic activity as Mel. A homogeneous liposome time-resolved fluoroimmunoassay (LITRFIA) of Th in serum has been carried out with Th-G1-Mel between 5 ng and 10 microg.

@article{citeulike:613250, abstract = {Theophylline (Th) has been selectively conjugated to the four amino groups of melittin (Mel) by solid phase peptide synthesis. The cytolytic activity of the resultant Th-Mel compounds was tested on liposomes trapping the bovine serum albumin (BSA) conjugate with 4,7-bis(chlorosulfophenyl)-1,10-phenanthrol ine-2,9-dicarboxylic acid (BCPDA). The loss of lytic activity was the highest for Th-K7-Mel. Th-G1-Mel retains almost the same lytic activity as Mel. A homogeneous liposome time-resolved fluoroimmunoassay (LITRFIA) of Th in serum has been carried out with Th-G1-Mel between 5 ng and 10 microg.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Istituto di Biocatalisi e Riconoscimento Molecolare, C.N.R., Milan, Italy. iusWico.mi.cnr.it}, author = {Ius, A. and Bacigalupo, M. A. and Longhi, R. and Meroni, G.}, biburl = {https://www.bibsonomy.org/bibtex/2da6a8fedfd5832158792638971ec8469/biblio24}, citeulike-article-id = {613250}, interhash = {bccf958d7fa048e8632771d801ef0a37}, intrahash = {da6a8fedfd5832158792638971ec8469}, issn = {0937-0633}, journal = {Fresenius J Anal Chem}, keywords = {lytic_peptide immunoassay liposome mellitin}, month = {April}, number = 8, pages = {869--872}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Selectively conjugated melittins for liposome time-resolved fluoroimmunoassay of theophylline in serum.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=11227424}, volume = 366, year = 2000 }