%0 Journal Article %1 citeulike:561064 %A Vrga, L. %A Contacos, C. %A Li, S. C. %A Sullivan, D. R. %C Department of Clinical Biochemistry, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia. %D 1997 %J Clin Chem %K cholesterol ldl apob %N 2 %P 390--393 %T Comparison of methods for measurement of apolipoprotein B and cholesterol in low-density lipoproteins. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9023145 %V 43 %X We describe a new method for the direct measurement of LDL-apolipoprotein (apo) B by using a commercial kit that isolates LDL by immunoseparation. We evaluated immunoseparation of LDL for apo B and cholesterol measurement in 46 dyslipidemic patients with LDL-cholesterol (LDL-C) between 1.5 and 8.2 mmol/L, 11 of whom had plasma triglyceride (TG) concentrations >4.0 mmol/L. There was a reasonable correlation (r = 0.94, n = 40) between LDL-apo B obtained after immunoseparation and d >1.006 kg/L apo B obtained after ultracentrifugation. LDL-C by the immunoseparation method also correlated well (r = 0.98, n = 46) with the d >1.006 kg/L cholesterol after ultracentrifugation. These results show that immunoseparation can be used to determine LDL-apo B, even in hypertriglyceridemic samples. This method may provide a quick and simple alternative for the identification of hyperapobetalipoproteinemia, even when TG concentrations are high.

@article{citeulike:561064, abstract = {We describe a new method for the direct measurement of LDL-apolipoprotein (apo) B by using a commercial kit that isolates LDL by immunoseparation. We evaluated immunoseparation of LDL for apo B and cholesterol measurement in 46 dyslipidemic patients with LDL-cholesterol (LDL-C) between 1.5 and 8.2 mmol/L, 11 of whom had plasma triglyceride (TG) concentrations >4.0 mmol/L. There was a reasonable correlation (r = 0.94, n = 40) between LDL-apo B obtained after immunoseparation and d >1.006 kg/L apo B obtained after ultracentrifugation. LDL-C by the immunoseparation method also correlated well (r = 0.98, n = 46) with the d >1.006 kg/L cholesterol after ultracentrifugation. These results show that immunoseparation can be used to determine LDL-apo B, even in hypertriglyceridemic samples. This method may provide a quick and simple alternative for the identification of hyperapobetalipoproteinemia, even when TG concentrations are high.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Clinical Biochemistry, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia.}, author = {Vrga, L. and Contacos, C. and Li, S. C. and Sullivan, D. R.}, biburl = {https://www.bibsonomy.org/bibtex/26070d1d106d2daf789f2cd667b27bfe4/biblio24}, citeulike-article-id = {561064}, interhash = {c4c0f8cf7e81769ffb53738d8040b180}, intrahash = {6070d1d106d2daf789f2cd667b27bfe4}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {cholesterol ldl apob}, month = {February}, number = 2, pages = {390--393}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Comparison of methods for measurement of apolipoprotein B and cholesterol in low-density lipoproteins.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9023145}, volume = 43, year = 1997 }