%0 Journal Article %1 topfQuantitativeProteomicsIdentifies2018a %A Topf, Ulrike %A Suppanz, Ida %A Samluk, Lukasz %A Wrobel, Lidia %A Böser, Alexander %A Sakowska, Paulina %A Knapp, Bettina %A Pietrzyk, Martyna K. %A Chacinska, Agnieszka %A Warscheid, Bettina %C England %D 2018 %J Nature communications %K Biosynthesis/*genetics,Ribosomal Cell Cells,Humans,Hydrogen Compounds/chemistry,to_read Line,HEK293 Peroxide/*metabolism,Mitochondria/*metabolism,Oxidation-Reduction,Oxidative Proteins/metabolism,Saccharomyces Stress/*physiology,Protein Transduction,Sulfhydryl cerevisiae/*metabolism,Signal %N 1 %P 324 %R 10.1038/s41467-017-02694-8 %T Quantitative Proteomics Identifies Redox Switches for Global Translation Modulation by Mitochondrially Produced Reactive Oxygen Species. %V 9 %X The generation of reactive oxygen species (ROS) is inevitably linked to life. However, the precise role of ROS in signalling and specific targets is largely unknown. We perform a global proteomic analysis to delineate the yeast redoxome to a depth of more than 4,300 unique cysteine residues in over 2,200 proteins. Mapping of redox-active thiols in proteins exposed to exogenous or endogenous mitochondria-derived oxidative stress reveals ROS-sensitive sites in several components of the translation apparatus. Mitochondria are the major source of cellular ROS. We demonstrate that increased levels of intracellular ROS caused by dysfunctional mitochondria serve as a signal to attenuate global protein synthesis. Hence, we propose a universal mechanism that controls protein synthesis by inducing reversible changes in the translation machinery upon modulating the redox status of proteins involved in translation. This crosstalk between mitochondria and protein synthesis may have an important contribution to pathologies caused by dysfunctional mitochondria.

@article{topfQuantitativeProteomicsIdentifies2018a, abstract = {The generation of reactive oxygen species (ROS) is inevitably linked to life. However, the precise role of ROS in signalling and specific targets is largely unknown. We perform a global proteomic analysis to delineate the yeast redoxome to a depth of more than 4,300 unique cysteine residues in over 2,200 proteins. Mapping of redox-active thiols in proteins exposed to exogenous or endogenous mitochondria-derived oxidative stress reveals ROS-sensitive sites in several components of the translation apparatus. Mitochondria are the major source of cellular ROS. We demonstrate that increased levels of intracellular ROS caused by dysfunctional mitochondria serve as a signal to attenuate global protein synthesis. Hence, we propose a universal mechanism that controls protein synthesis by inducing reversible changes in the translation machinery upon modulating the redox status of proteins involved in translation. This crosstalk between mitochondria and protein synthesis may have an important contribution to pathologies caused by dysfunctional mitochondria.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {England}, author = {Topf, Ulrike and Suppanz, Ida and Samluk, Lukasz and Wrobel, Lidia and B{\"o}ser, Alexander and Sakowska, Paulina and Knapp, Bettina and Pietrzyk, Martyna K. and Chacinska, Agnieszka and Warscheid, Bettina}, biburl = {https://www.bibsonomy.org/bibtex/2ae21d4bf8d0bf875f8a7f7a66bd3b63d/warscheidlab}, doi = {10.1038/s41467-017-02694-8}, interhash = {c74707787e228cb22df7710557c0d76a}, intrahash = {ae21d4bf8d0bf875f8a7f7a66bd3b63d}, issn = {2041-1723}, journal = {Nature communications}, keywords = {Biosynthesis/*genetics,Ribosomal Cell Cells,Humans,Hydrogen Compounds/chemistry,to_read Line,HEK293 Peroxide/*metabolism,Mitochondria/*metabolism,Oxidation-Reduction,Oxidative Proteins/metabolism,Saccharomyces Stress/*physiology,Protein Transduction,Sulfhydryl cerevisiae/*metabolism,Signal}, langid = {english}, month = jan, number = 1, pages = 324, pmcid = {PMC5778013}, pmid = {29358734}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Quantitative Proteomics Identifies Redox Switches for Global Translation Modulation by Mitochondrially Produced Reactive Oxygen Species.}, volume = 9, year = 2018 }