%0 Journal Article %1 10.1371/journal.pone.0057860 %A Arunasri, Kotakonda %A Adil, Mohammed %A Venu Charan, Katari %A Suvro, Chatterjee %A Himabindu Reddy, Seerapu %A Shivaji, Sisinthy %D 2013 %I Public Library of Science %J PLoS ONE %K 8ecoli %N 3 %P e57860 %R 10.1371/journal.pone.0057860 %T Effect of Simulated Microgravity on <italic>E. coli</italic> K12 MG1655 Growth and Gene Expression %U http://dx.doi.org/10.1371%2Fjournal.pone.0057860 %V 8 %X <p>This study demonstrates the effects of simulated microgravity on <italic>E. coli</italic> K 12 MG1655 grown on LB medium supplemented with glycerol. Global gene expression analysis indicated that the expressions of hundred genes were significantly altered in simulated microgravity conditions compared to that of normal gravity conditions. Under these conditions genes coding for adaptation to stress are up regulated (<italic>sufE</italic> and <italic>ssrA</italic>) and simultaneously genes coding for membrane transporters (<italic>ompC, exbB, actP, mgtA, cysW</italic> and <italic>nikB</italic>) and carbohydrate catabolic processes (<italic>ldcC, ptsA, rhaD</italic> and <italic>rhaS</italic>) are down regulated. The enhanced growth in simulated gravity conditions may be because of the adequate supply of energy/reducing equivalents and up regulation of genes involved in DNA replication (<italic>srmB</italic>) and repression of the genes encoding for nucleoside metabolism (<italic>dfp, pyrD</italic> and <italic>spoT</italic>). In addition, <italic>E. coli</italic> cultured in LB medium supplemented with glycerol (so as to protect the cells from freezing temperatures) do not exhibit multiple stress responses that are normally observed when cells are exposed to microgravity in LB medium without glycerol.</p>

@article{10.1371/journal.pone.0057860, abstract = {<p>This study demonstrates the effects of simulated microgravity on <italic>E. coli</italic> K 12 MG1655 grown on LB medium supplemented with glycerol. Global gene expression analysis indicated that the expressions of hundred genes were significantly altered in simulated microgravity conditions compared to that of normal gravity conditions. Under these conditions genes coding for adaptation to stress are up regulated (<italic>sufE</italic> and <italic>ssrA</italic>) and simultaneously genes coding for membrane transporters (<italic>ompC, exbB, actP, mgtA, cysW</italic> and <italic>nikB</italic>) and carbohydrate catabolic processes (<italic>ldcC, ptsA, rhaD</italic> and <italic>rhaS</italic>) are down regulated. The enhanced growth in simulated gravity conditions may be because of the adequate supply of energy/reducing equivalents and up regulation of genes involved in DNA replication (<italic>srmB</italic>) and repression of the genes encoding for nucleoside metabolism (<italic>dfp, pyrD</italic> and <italic>spoT</italic>). In addition, <italic>E. coli</italic> cultured in LB medium supplemented with glycerol (so as to protect the cells from freezing temperatures) do not exhibit multiple stress responses that are normally observed when cells are exposed to microgravity in LB medium without glycerol.</p>}, added-at = {2014-01-30T18:30:25.000+0100}, author = {Arunasri, Kotakonda and Adil, Mohammed and Venu Charan, Katari and Suvro, Chatterjee and Himabindu Reddy, Seerapu and Shivaji, Sisinthy}, biburl = {https://www.bibsonomy.org/bibtex/26d355f98b7f624a4c2394d81e3304106/emmamryan}, description = {PLOS ONE: Effect of Simulated Microgravity on E. coli K12 MG1655 Growth and Gene Expression}, doi = {10.1371/journal.pone.0057860}, interhash = {cbec7a8e34680158749a5d12e1e51044}, intrahash = {6d355f98b7f624a4c2394d81e3304106}, journal = {PLoS ONE}, keywords = {8ecoli}, month = {03}, number = 3, pages = {e57860}, publisher = {Public Library of Science}, timestamp = {2014-01-30T18:30:25.000+0100}, title = {Effect of Simulated Microgravity on <italic>E. coli</italic> K12 MG1655 Growth and Gene Expression}, url = {http://dx.doi.org/10.1371%2Fjournal.pone.0057860}, volume = 8, year = 2013 }