Zusammenfassung
The viscoelastic properties of human kidney cell lines from different
tumor types (carcinoma (A-498) and adenocarcinoma (ACHN)) are compared
to a non-tumorigenic cell line (RC-124). Our methodology is based on the
mapping of viscoelastic properties (elasticity modulus E and apparent
viscosity eta) over the surface of tens of individual cells with atomic
force microscopy (AFM). The viscoelastic properties are averaged over
datasets as large as 15000 data points per cell line. We also propose a
model to estimate the apparent viscosity of soft materials using the
hysteresis observed in conventional AFM deflection-displacement curves,
without any modification to the standard AFM apparatus. The comparison
of the three cell lines show that the non-tumorigenic cells are less
deformable and more viscous than cancerous cells, and that cancer cell
lines have distinctive viscoelastic properties. In particular, we
obtained that ERC-124 > EA-498 > E-ACHN and eta(RC-124) > eta(A-498) >
eta(ACHN).
Nutzer