%0 Journal Article %1 Cai06 %A Cai, Shutao %A Lee, Charles C %A Kohwi-Shigematsu, Terumi %D 2006 %J Nat. Genet. %K Acetylation Animals Binding_Sites Chromatin,_chemistry/metabolism Chromatin_Immunoprecipitation,_methods Chromosomes,_chemistry Cytokines,_metabolism DNA-Binding_Proteins,_genetics/physiology DNA_Helicases,_metabolism DNA_Repair_Enzymes,_genetics GATA3_Transcription_Factor,_metabolism Gene_Expression_Regulation Histone_Acetyltransferases,_metabolism Histones,_metabolism Locus_Control_Region Lymphocyte_Activation,_physiology Matrix_Attachment_Region_Binding_Proteins,_physiology Mice Mice,_Inbred_AKR Models,_Biological Nuclear_Proteins,_metabolism Nucleic_Acid_Conformation Promoter_Regions_(Genetics) Proto-Oncogene_Proteins_c-maf,_metabolism RNA_Polymerase_II,_metabolism STAT6_Transcription_Factor,_metabolism Th2_Cells,_metabolism Transcription,_Genetic,_physiology Transcription_Factors,_metabolism %N 11 %P 1278-88 %R 10.1038/ng1913 %T SATB1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes. %U http://dx.doi.org/10.1038/ng1913 %V 38 %X SATB1 (special AT-rich sequence binding protein 1) organizes cell type-specific nuclear architecture by anchoring specialized DNA sequences and recruiting chromatin remodeling factors to control gene transcription. We studied the role of SATB1 in regulating the coordinated expression of Il5, Il4 and Il13, located in the 200-kb T-helper 2 (T(H)2) cytokine locus on mouse chromosome 11. We show that on T(H)2 cell activation, SATB1 expression is rapidly induced to form a unique transcriptionally active chromatin structure at the cytokine locus. In this structure, chromatin is folded into numerous small loops, all anchored to SATB1 at their base. In addition, histone H3 is acetylated at Lys9 and Lys14, and the T(H)2-specific factors GATA3, STAT6 and c-Maf, the chromatin-remodeling enzyme Brg1 and RNA polymerase II are all bound across the 200-kb region. Before activation, the T(H)2 cytokine locus is already associated with GATA3 and STAT6, showing some looping, but these are insufficient to induce cytokine gene expression. Using RNA interference, we show that on cell activation, SATB1 is required not only for compacting chromatin into dense loops at the 200-kb cytokine locus but also for inducing Il4, Il5, Il13 and c-Maf expression. Thus, SATB1 is a necessary determinant for the hitherto unidentified higher-order, transcriptionally active chromatin structure that forms on T(H)2 cell activation.

@article{Cai06, abstract = {SATB1 (special AT-rich sequence binding protein 1) organizes cell type-specific nuclear architecture by anchoring specialized DNA sequences and recruiting chromatin remodeling factors to control gene transcription. We studied the role of SATB1 in regulating the coordinated expression of Il5, Il4 and Il13, located in the 200-kb T-helper 2 (T(H)2) cytokine locus on mouse chromosome 11. We show that on T(H)2 cell activation, SATB1 expression is rapidly induced to form a unique transcriptionally active chromatin structure at the cytokine locus. In this structure, chromatin is folded into numerous small loops, all anchored to SATB1 at their base. In addition, histone H3 is acetylated at Lys9 and Lys14, and the T(H)2-specific factors GATA3, STAT6 and c-Maf, the chromatin-remodeling enzyme Brg1 and RNA polymerase II are all bound across the 200-kb region. Before activation, the T(H)2 cytokine locus is already associated with GATA3 and STAT6, showing some looping, but these are insufficient to induce cytokine gene expression. Using RNA interference, we show that on cell activation, SATB1 is required not only for compacting chromatin into dense loops at the 200-kb cytokine locus but also for inducing Il4, Il5, Il13 and c-Maf expression. Thus, SATB1 is a necessary determinant for the hitherto unidentified higher-order, transcriptionally active chromatin structure that forms on T(H)2 cell activation.}, added-at = {2010-01-26T20:35:53.000+0100}, author = {Cai, Shutao and Lee, Charles C and Kohwi-Shigematsu, Terumi}, biburl = {https://www.bibsonomy.org/bibtex/2688e42c1312a37a9813e2bc9cf489b1c/denilw}, doi = {10.1038/ng1913}, file = {article:../Transcription Factor Binding/SatB1/SATB1 packages densely looped%2C transcriptionally active chromatin for coordinated expression of cytokine genes.pdf:pdf}, institution = {Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720, USA.}, interhash = {d5bb3e6c8cb7f4fdb0f7545d2da94c98}, intrahash = {688e42c1312a37a9813e2bc9cf489b1c}, journal = {Nat. Genet.}, keywords = {Acetylation Animals Binding_Sites Chromatin,_chemistry/metabolism Chromatin_Immunoprecipitation,_methods Chromosomes,_chemistry Cytokines,_metabolism DNA-Binding_Proteins,_genetics/physiology DNA_Helicases,_metabolism DNA_Repair_Enzymes,_genetics GATA3_Transcription_Factor,_metabolism Gene_Expression_Regulation Histone_Acetyltransferases,_metabolism Histones,_metabolism Locus_Control_Region Lymphocyte_Activation,_physiology Matrix_Attachment_Region_Binding_Proteins,_physiology Mice Mice,_Inbred_AKR Models,_Biological Nuclear_Proteins,_metabolism Nucleic_Acid_Conformation Promoter_Regions_(Genetics) Proto-Oncogene_Proteins_c-maf,_metabolism RNA_Polymerase_II,_metabolism STAT6_Transcription_Factor,_metabolism Th2_Cells,_metabolism Transcription,_Genetic,_physiology Transcription_Factors,_metabolism}, month = Nov, number = 11, owner = {denilw}, pages = {1278-88}, pii = {ng1913}, pmid = {17057718}, timestamp = {2010-01-26T20:35:56.000+0100}, title = {SATB1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes.}, url = {http://dx.doi.org/10.1038/ng1913}, volume = 38, year = 2006 }