%0 Journal Article %1 vogg1999sensitive %A Vogg, Gerd %A Achatz, S %A Kettrup, A %A Sandermann Jr., Heinrich %D 1999 %J Journal of Chromatography A %K myown %N 2 %P 563-573 %T Fast, sensitive and selective liquid chromatographic-tandem mass spectrometric determination of tumor-promoting diterpene eaters %U http://dx.doi.org/10.1016/S0021-9673(99)00728-1 %V 855 %X A liquid chromatography–tandem mass spectrometry (LC–MS–MS) method was developed to detect tumor-promoting diterpene esters of the tigliane and ingenane types within plant extracts. Fractionation on a C18 high-performance liquid chromatography (HPLC) column was followed by MS–MS–multiple reaction monitoring (MRM) using the precursor→product ion pairs of m/z 311→293 and 293→265 for phorbol esters. The ion pairs m/z 313→295 and 295→267 were used for ingenol and deoxyphorbol esters. In a second run, the characteristic ions at m/z 311 and 313 were followed in precursor ion scan mode. These quasi-molecular ions were utilized to obtain full scan spectra of the compounds in product ion scan mode. Due to its selectivity, the present on-line method can be applied for plant cultivar selection and plant product control without time-consuming extraction procedures and complex bioassays.

@article{vogg1999sensitive, abstract = {A liquid chromatography–tandem mass spectrometry (LC–MS–MS) method was developed to detect tumor-promoting diterpene esters of the tigliane and ingenane types within plant extracts. Fractionation on a C18 high-performance liquid chromatography (HPLC) column was followed by MS–MS–multiple reaction monitoring (MRM) using the precursor→product ion pairs of m/z 311→293 and 293→265 for phorbol esters. The ion pairs m/z 313→295 and 295→267 were used for ingenol and deoxyphorbol esters. In a second run, the characteristic ions at m/z 311 and 313 were followed in precursor ion scan mode. These quasi-molecular ions were utilized to obtain full scan spectra of the compounds in product ion scan mode. Due to its selectivity, the present on-line method can be applied for plant cultivar selection and plant product control without time-consuming extraction procedures and complex bioassays.}, added-at = {2013-03-15T14:10:31.000+0100}, author = {Vogg, Gerd and Achatz, S and Kettrup, A and Sandermann Jr., Heinrich}, biburl = {https://www.bibsonomy.org/bibtex/29fede2059a37d682fb4ae272520b4685/vogg}, interhash = {e3bca891f90b4b258cb3a43b9eedc163}, intrahash = {9fede2059a37d682fb4ae272520b4685}, journal = {Journal of Chromatography A}, keywords = {myown}, month = sep, number = 2, pages = {563-573}, timestamp = {2013-03-15T14:28:05.000+0100}, title = {Fast, sensitive and selective liquid chromatographic-tandem mass spectrometric determination of tumor-promoting diterpene eaters}, url = {http://dx.doi.org/10.1016/S0021-9673(99)00728-1}, volume = 855, year = 1999 }