%0 Journal Article %1 gebertMgr2PromotesCoupling2012a %A Gebert, Michael %A Schrempp, Sandra G. %A Mehnert, Carola S. %A Heißwolf, Anna K. %A Oeljeklaus, Silke %A Ieva, Raffaele %A Bohnert, Maria %A von der Malsburg, Karina %A Wiese, Sebastian %A Kleinschroth, Thomas %A Hunte, Carola %A Meyer, Helmut E. %A Haferkamp, Ilka %A Guiard, Bernard %A Warscheid, Bettina %A Pfanner, Nikolaus %A van der Laan, Martin %C United States %D 2012 %J The Journal of cell biology %K & Cell Complex Complexes/chemistry/*metabolism,Protein Import Membrane Precursor Proliferation,Membrane Protein Proteins,Multiprotein Proteins/*metabolism,Membrane Proteins/chemistry/*metabolism,Mitochondrial Proteins/chemistry/*metabolism,Saccharomyces Subunits/*metabolism,Protein Transport Transport,Saccharomyces cerevisiae cerevisiae/cytology/growth development/metabolism,Temperature,to_read %N 5 %P 595--604 %R 10.1083/jcb.201110047 %T Mgr2 Promotes Coupling of the Mitochondrial Presequence Translocase to Partner Complexes. %V 197 %X Many mitochondrial proteins are synthesized with N-terminal presequences in the cytosol. The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor. The TIM23 complex consists of three core components and Tim21, which interacts with the translocase of the outer membrane (TOM) and the respiratory chain. We have identified a new subunit of the TIM23 complex, the inner membrane protein Mgr2. Mitochondria lacking Mgr2 were deficient in the Tim21-containing sorting form of the TIM23 complex. Mgr2 was required for binding of Tim21 to TIM23(CORE), revealing a binding chain of TIM23(CORE)-Mgr2/Tim21-respiratory chain. Mgr2-deficient yeast cells were defective in growth at elevated temperature, and the mitochondria were impaired in TOM-TIM23 coupling and the import of presequence-carrying preproteins. We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import.

@article{gebertMgr2PromotesCoupling2012a, abstract = {Many mitochondrial proteins are synthesized with N-terminal presequences in the cytosol. The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor. The TIM23 complex consists of three core components and Tim21, which interacts with the translocase of the outer membrane (TOM) and the respiratory chain. We have identified a new subunit of the TIM23 complex, the inner membrane protein Mgr2. Mitochondria lacking Mgr2 were deficient in the Tim21-containing sorting form of the TIM23 complex. Mgr2 was required for binding of Tim21 to TIM23(CORE), revealing a binding chain of TIM23(CORE)-Mgr2/Tim21-respiratory chain. Mgr2-deficient yeast cells were defective in growth at elevated temperature, and the mitochondria were impaired in TOM-TIM23 coupling and the import of presequence-carrying preproteins. We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {United States}, author = {Gebert, Michael and Schrempp, Sandra G. and Mehnert, Carola S. and Hei{\ss}wolf, Anna K. and Oeljeklaus, Silke and Ieva, Raffaele and Bohnert, Maria and {von der Malsburg}, Karina and Wiese, Sebastian and Kleinschroth, Thomas and Hunte, Carola and Meyer, Helmut E. and Haferkamp, Ilka and Guiard, Bernard and Warscheid, Bettina and Pfanner, Nikolaus and {van der Laan}, Martin}, biburl = {https://www.bibsonomy.org/bibtex/2d40d3bda1aeb87f5da32aec8eb58cd34/warscheidlab}, doi = {10.1083/jcb.201110047}, interhash = {e94117583114b2c36da9cf3cc5344db7}, intrahash = {d40d3bda1aeb87f5da32aec8eb58cd34}, issn = {1540-8140 0021-9525}, journal = {The Journal of cell biology}, keywords = {& Cell Complex Complexes/chemistry/*metabolism,Protein Import Membrane Precursor Proliferation,Membrane Protein Proteins,Multiprotein Proteins/*metabolism,Membrane Proteins/chemistry/*metabolism,Mitochondrial Proteins/chemistry/*metabolism,Saccharomyces Subunits/*metabolism,Protein Transport Transport,Saccharomyces cerevisiae cerevisiae/cytology/growth development/metabolism,Temperature,to_read}, langid = {english}, month = may, number = 5, pages = {595--604}, pmcid = {PMC3365495}, pmid = {22613836}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Mgr2 Promotes Coupling of the Mitochondrial Presequence Translocase to Partner Complexes.}, volume = 197, year = 2012 }