%0 Journal Article %1 EMI:EMI13746 %A Bill, Nelli %A Tomasch, Jürgen %A Riemer, Alexander %A Müller, Katrin %A Kleist, Sarah %A Schmidt-Hohagen, Kerstin %A Wagner-Döbler, Irene %A Schomburg, Dietmar %D 2017 %J Environmental Microbiology %K Dinoroseobacter ethylmalonyl-CoA myown pathway shibae %N 7 %P 2645--2660 %R 10.1111/1462-2920.13746 %T Fixation of CO2 using the ethylmalonyl-CoA pathway in the photoheterotrophic marine bacterium Dinoroseobacter shibae %U http://dx.doi.org/10.1111/1462-2920.13746 %V 19 %X The ability of aerobic anoxygenic photoheterotrophs (AAPs) to gain additional energy from sunlight represents a competitive advantage, especially in conditions where light has easy access or under environmental conditions may change quickly, such as those in the world´s oceans. However, the knowledge about the metabolic consequences of aerobic anoxygenic photosynthesis is very limited. Combining transcriptome and metabolome analyses, isotopic labelling techniques, measurements of growth, oxygen uptake rates, flow cytometry, and a number of other biochemical analytical techniques we obtained a comprehensive overview on the complex adaption of the marine bacterium Dinoroseobacter shibae DFL12T during transition from heterotrophy to photoheterotrophy (growth on succinate). Growth in light was characterized by reduced respiration, a decreased metabolic flux through the tricarboxylic acid (TCA) cycle and the assimilation of CO2 via an enhanced flux through the ethylmalonyl-CoA (EMC) pathway, which was shown to be connected to the serine metabolism. Adaptation to photoheterotrophy is mainly characterized by metabolic reactions caused by a surplus of reducing potential and might depend on genes located in one operon, encoding branching point enzymes of the EMC pathway, serine metabolism and the TCA cycle.

@article{EMI:EMI13746, abstract = {The ability of aerobic anoxygenic photoheterotrophs (AAPs) to gain additional energy from sunlight represents a competitive advantage, especially in conditions where light has easy access or under environmental conditions may change quickly, such as those in the world´s oceans. However, the knowledge about the metabolic consequences of aerobic anoxygenic photosynthesis is very limited. Combining transcriptome and metabolome analyses, isotopic labelling techniques, measurements of growth, oxygen uptake rates, flow cytometry, and a number of other biochemical analytical techniques we obtained a comprehensive overview on the complex adaption of the marine bacterium Dinoroseobacter shibae DFL12T during transition from heterotrophy to photoheterotrophy (growth on succinate). Growth in light was characterized by reduced respiration, a decreased metabolic flux through the tricarboxylic acid (TCA) cycle and the assimilation of CO2 via an enhanced flux through the ethylmalonyl-CoA (EMC) pathway, which was shown to be connected to the serine metabolism. Adaptation to photoheterotrophy is mainly characterized by metabolic reactions caused by a surplus of reducing potential and might depend on genes located in one operon, encoding branching point enzymes of the EMC pathway, serine metabolism and the TCA cycle.}, added-at = {2017-07-27T09:24:54.000+0200}, author = {Bill, Nelli and Tomasch, Jürgen and Riemer, Alexander and Müller, Katrin and Kleist, Sarah and Schmidt-Hohagen, Kerstin and Wagner-Döbler, Irene and Schomburg, Dietmar}, biburl = {https://www.bibsonomy.org/bibtex/2dedb9eb0c9fcd6e11ee1a7bd99f57bd0/brenda_bs}, doi = {10.1111/1462-2920.13746}, interhash = {fcda17c58269e130e1110f8247e345aa}, intrahash = {dedb9eb0c9fcd6e11ee1a7bd99f57bd0}, issn = {1462-2920}, journal = {Environmental Microbiology}, keywords = {Dinoroseobacter ethylmalonyl-CoA myown pathway shibae}, number = 7, pages = {2645--2660}, timestamp = {2017-10-30T09:32:00.000+0100}, title = {Fixation of CO2 using the ethylmalonyl-CoA pathway in the photoheterotrophic marine bacterium Dinoroseobacter shibae}, url = {http://dx.doi.org/10.1111/1462-2920.13746}, volume = 19, year = 2017 }