This study sought to investigate the relationship existing between laboratory facility availability and students’ academic performance in Biology across Secondary School in Ethiope East L.G.A., of Delta State, Nigeria. In the course of this study, three research questions were developed and three null hypotheses were formulated. The study used was delimited to senior secondary schools two (SSII) because it is at this stage students are being exposed to practical activities that require the usage of laboratory facilities and equipment. A descriptive survey design was used while the sample of the study was made up of one hundred and three (103) respondents. The study employed a questionnaire as an instrument to gather data.