%0 Journal Article %1 RN1254 %A Zimmermann, S. %A Nurnberger, T. %A Frachisse, J. M. %A Wirtz, W. %A Guern, J. %A Hedrich, R. %A Scheel, D. %D 1997 %J Proceedings of the National Academy of Sciences of the United States of America %K myOwn patch-clamp %N 6 %P 2751-2755 %R DOI 10.1073/pnas.94.6.2751 %T Receptor-mediated activation of a plant Ca2+-permeable ion channel involved in pathogen defense %U /brokenurl#<Go to ISI>://WOS:A1997WP33400120 %V 94 %X Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+ across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance, Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+- sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2), At an extracellular Ca2+ concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS, This ion channel (LEAC, for large conductance elicitor-activated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae, Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC, Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley.

@article{RN1254, abstract = {Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+ across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance, Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+- sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2), At an extracellular Ca2+ concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS, This ion channel (LEAC, for large conductance elicitor-activated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae, Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC, Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Zimmermann, S. and Nurnberger, T. and Frachisse, J. M. and Wirtz, W. and Guern, J. and Hedrich, R. and Scheel, D.}, biburl = {https://www.bibsonomy.org/bibtex/21bfd45f31e626e5f03611ae1e1005e26/rainerhedrich_2}, doi = {DOI 10.1073/pnas.94.6.2751}, interhash = {d5c0a83ff56372e3cec1b44f65fc5bf3}, intrahash = {1bfd45f31e626e5f03611ae1e1005e26}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {myOwn patch-clamp}, note = {Wp334 Times Cited:174 Cited References Count:41}, number = 6, pages = {2751-2755}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Receptor-mediated activation of a plant Ca2+-permeable ion channel involved in pathogen defense}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:A1997WP33400120}, volume = 94, year = 1997 }