Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+ across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance, Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+- sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2), At an extracellular Ca2+ concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS, This ion channel (LEAC, for large conductance elicitor-activated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae, Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC, Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley.
%0 Journal Article
%1 RN1254
%A Zimmermann, S.
%A Nurnberger, T.
%A Frachisse, J. M.
%A Wirtz, W.
%A Guern, J.
%A Hedrich, R.
%A Scheel, D.
%D 1997
%J Proceedings of the National Academy of Sciences of the United States of America
%K myOwn patch-clamp
%N 6
%P 2751-2755
%R DOI 10.1073/pnas.94.6.2751
%T Receptor-mediated activation of a plant Ca2+-permeable ion channel involved in pathogen defense
%U /brokenurl#<Go to ISI>://WOS:A1997WP33400120
%V 94
%X Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+ across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance, Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+- sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2), At an extracellular Ca2+ concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS, This ion channel (LEAC, for large conductance elicitor-activated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae, Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC, Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley.
@article{RN1254,
abstract = {Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+ across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance, Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+- sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2), At an extracellular Ca2+ concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS, This ion channel (LEAC, for large conductance elicitor-activated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae, Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC, Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Zimmermann, S. and Nurnberger, T. and Frachisse, J. M. and Wirtz, W. and Guern, J. and Hedrich, R. and Scheel, D.},
biburl = {https://www.bibsonomy.org/bibtex/21bfd45f31e626e5f03611ae1e1005e26/rainerhedrich_2},
doi = {DOI 10.1073/pnas.94.6.2751},
interhash = {d5c0a83ff56372e3cec1b44f65fc5bf3},
intrahash = {1bfd45f31e626e5f03611ae1e1005e26},
issn = {0027-8424},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
keywords = {myOwn patch-clamp},
note = {Wp334
Times Cited:174
Cited References Count:41},
number = 6,
pages = {2751-2755},
timestamp = {2024-02-14T14:38:32.000+0100},
title = {Receptor-mediated activation of a plant Ca2+-permeable ion channel involved in pathogen defense},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:A1997WP33400120},
volume = 94,
year = 1997
}