%0 Journal Article %1 WOS:000231909100001 %A Farias, PMA De %A Santos, BS %A Menezes, FD De %A Ferreira, RD %A Barjas-Castro, MD %A Castro, V %A Lima, PRM %A Fontes, A %A Cesar, CL %C 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %D 2005 %I WILEY %J JOURNAL OF MICROSCOPY %K biophotonics; blood cell} dots; fluorescent markers; quantum red {bioconjugation; %N 3 %P 103-108 %T Core-shell CdS/Cd(OH)(2) quantum dots: synthesis and bioconjugation to target red cells antigens %V 219 %X We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti-A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core-shell cadmium sulphide/cadmium hydroxide CdS/CdS(OH)(2) colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti-A antibody via a one-step glutaraldehyde cross-linking procedure, followed by conjugation of the particles to red cells of blood groups A(+), A(2)(+) and O+. Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A(+) and A(2)(+) erythrocytes presented different patterns of dual bright emission whereas the O+ group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells.

@article{WOS:000231909100001, abstract = {We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti-A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core-shell cadmium sulphide/cadmium hydroxide [CdS/CdS(OH)(2)] colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti-A antibody via a one-step glutaraldehyde cross-linking procedure, followed by conjugation of the particles to red cells of blood groups A(+), A(2)(+) and O+. Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A(+) and A(2)(+) erythrocytes presented different patterns of dual bright emission whereas the O+ group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA}, author = {Farias, PMA De and Santos, BS and Menezes, FD De and Ferreira, RD and Barjas-Castro, MD and Castro, V and Lima, PRM and Fontes, A and Cesar, CL}, biburl = {https://www.bibsonomy.org/bibtex/2d2a1afda56d92a69be103b240d229de9/ppgfis_ufc_br}, interhash = {70ab72be8f78acaf0f69fe819cca30f5}, intrahash = {d2a1afda56d92a69be103b240d229de9}, issn = {0022-2720}, journal = {JOURNAL OF MICROSCOPY}, keywords = {biophotonics; blood cell} dots; fluorescent markers; quantum red {bioconjugation;}, number = 3, pages = {103-108}, publisher = {WILEY}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Core-shell CdS/Cd(OH)(2) quantum dots: synthesis and bioconjugation to target red cells antigens}, tppubtype = {article}, volume = 219, year = 2005 }