%0 Journal Article %1 Dunkel:2008:Mol-Plant:19825594 %A Dunkel, M %A Latz, A %A Schumacher, K %A Müller, T %A Becker, D %A Hedrich, R %D 2008 %J Mol Plant %K myown %N 6 %P 938-949 %R 10.1093/mp/ssn064 %T Targeting of vacuolar membrane localized members of the TPK channel family %U https://www.ncbi.nlm.nih.gov/pubmed/19825594 %V 1 %X Four members of the tandem-pore potassium channel family of Arabidopsis thaliana (TPK1, 2, 3, and 5) reside in the vacuolar membrane, whereas TPK4 is a plasma membrane K(+)-channel. By constructing chimeras between TPK1 and TPK4, we attempted to identify channel domains involved in the trafficking process and found that the TPK1 cytoplasmic C-terminal domain (CT) is critical for the ER- as well as Golgi-sorting steps. Following site-directed mutagenesis, we identified a diacidic motif (DLE) required for ER-export of TPK1. However, this diacidic motif in the C-terminus is not conserved among other members of the TPK family, and TPK3 sorting is independent of its CT. Moreover, the 14-3-3 binding site of TPK1, essential for channel activation, is not involved in channel sorting.

@article{Dunkel:2008:Mol-Plant:19825594, abstract = {Four members of the tandem-pore potassium channel family of Arabidopsis thaliana (TPK1, 2, 3, and 5) reside in the vacuolar membrane, whereas TPK4 is a plasma membrane K(+)-channel. By constructing chimeras between TPK1 and TPK4, we attempted to identify channel domains involved in the trafficking process and found that the TPK1 cytoplasmic C-terminal domain (CT) is critical for the ER- as well as Golgi-sorting steps. Following site-directed mutagenesis, we identified a diacidic motif (DLE) required for ER-export of TPK1. However, this diacidic motif in the C-terminus is not conserved among other members of the TPK family, and TPK3 sorting is independent of its CT. Moreover, the 14-3-3 binding site of TPK1, essential for channel activation, is not involved in channel sorting.}, added-at = {2017-03-21T20:38:05.000+0100}, author = {Dunkel, M and Latz, A and Schumacher, K and M{\"u}ller, T and Becker, D and Hedrich, R}, biburl = {https://www.bibsonomy.org/bibtex/2f1637a875272f7d32e87758bfbecd459/dirkbecker}, description = {Targeting of vacuolar membrane localized members of the TPK channel family. - PubMed - NCBI}, doi = {10.1093/mp/ssn064}, interhash = {49ec5206bb85cff99e91e3b103fb187d}, intrahash = {f1637a875272f7d32e87758bfbecd459}, journal = {Mol Plant}, keywords = {myown}, month = nov, number = 6, pages = {938-949}, pmid = {19825594}, timestamp = {2017-03-21T20:38:05.000+0100}, title = {Targeting of vacuolar membrane localized members of the TPK channel family}, url = {https://www.ncbi.nlm.nih.gov/pubmed/19825594}, volume = 1, year = 2008 }