%0 Journal Article %1 lappann_dual_2010 %A Lappann, Martin %A Claus, Heike %A van Alen, Tessa %A Harmsen, Morten %A Elias, Johannes %A Molin, Søren %A Vogel, Ulrich %D 2010 %J Molecular Microbiology %K Amidase, Bacterial Bacterial, Bacteriolysis, Biofilms, Glycosyltransferases, Neisseria Phospholipases Proteins, ag_vogel meningitidis, {DNA,} {N-Acetylmuramoyl-L-alanine} %N 6 %P 1355--1371 %R 10.1111/j.1365-2958.2010.07054.x %T A dual role of extracellular DNA during biofilm formation of Neisseria meningitidis %U http://www.ncbi.nlm.nih.gov/pubmed/20180907 %V 75 %X Major pathogenic clonal complexes (cc) of Neisseria meningitidis differ substantially in their point prevalence among healthy carriers. We show that frequently carried pathogenic cc (e.g. sequence type ST-41/44 cc and ST-32 cc) depend on extracellular DNA (eDNA) to initiate in vitro biofilm formation, whereas biofilm formation of cc with low point prevalence (ST-8 cc and ST-11 cc) was eDNA-independent. For initial biofilm formation, a ST-32 cc type strain, but not a ST-11 type strain, utilized eDNA. The release of eDNA was mediated by lytic transglycosylase and cytoplasmic N-acetylmuramyl-L-alanine amidase genes. In late biofilms, outer membrane phospholipase A-dependent autolysis, which was observed in most cc, but not in ST-8 and ST-11 strains, was required for shear force resistance of microcolonies. Taken together, N. meningitidis evolved two different biofilm formation strategies, an eDNA-dependent one yielding shear force resistant microcolonies, and an eDNA-independent one. Based on the experimental findings and previous epidemiological observations, we hypothesize that most meningococcal cc display a settler phenotype, which is eDNA-dependent and results in a stable interaction with the host. On the contrary, spreaders (ST-11 and ST-8 cc) are unable to use eDNA for biofilm formation and might compensate for poor colonization properties by high transmission rates.

@article{lappann_dual_2010, abstract = {Major pathogenic clonal complexes (cc) of Neisseria meningitidis differ substantially in their point prevalence among healthy carriers. We show that frequently carried pathogenic cc (e.g. sequence type {ST-41/44} cc and {ST-32} cc) depend on extracellular {DNA} {(eDNA)} to initiate in vitro biofilm formation, whereas biofilm formation of cc with low point prevalence {(ST-8} cc and {ST-11} cc) was {eDNA-independent.} For initial biofilm formation, a {ST-32} cc type strain, but not a {ST-11} type strain, utilized {eDNA.} The release of {eDNA} was mediated by lytic transglycosylase and cytoplasmic {N-acetylmuramyl-L-alanine} amidase genes. In late biofilms, outer membrane phospholipase A-dependent autolysis, which was observed in most cc, but not in {ST-8} and {ST-11} strains, was required for shear force resistance of microcolonies. Taken together, N. meningitidis evolved two different biofilm formation strategies, an {eDNA-dependent} one yielding shear force resistant microcolonies, and an {eDNA-independent} one. Based on the experimental findings and previous epidemiological observations, we hypothesize that most meningococcal cc display a settler phenotype, which is {eDNA-dependent} and results in a stable interaction with the host. On the contrary, spreaders {(ST-11} and {ST-8} cc) are unable to use {eDNA} for biofilm formation and might compensate for poor colonization properties by high transmission rates.}, added-at = {2011-04-07T15:44:20.000+0200}, author = {Lappann, Martin and Claus, Heike and van Alen, Tessa and Harmsen, Morten and Elias, Johannes and Molin, Søren and Vogel, Ulrich}, biburl = {https://www.bibsonomy.org/bibtex/21931f84f6fe6af080813760dc362c063/hymi}, doi = {10.1111/j.1365-2958.2010.07054.x}, interhash = {74e2fdb4e8517b8f32ff03f5efae76c0}, intrahash = {1931f84f6fe6af080813760dc362c063}, issn = {1365-2958}, journal = {Molecular Microbiology}, keywords = {Amidase, Bacterial Bacterial, Bacteriolysis, Biofilms, Glycosyltransferases, Neisseria Phospholipases Proteins, ag_vogel meningitidis, {DNA,} {N-Acetylmuramoyl-L-alanine}}, month = mar, note = {{PMID:} 20180907}, number = 6, pages = {1355--1371}, timestamp = {2011-04-07T16:28:36.000+0200}, title = {A dual role of extracellular {DNA} during biofilm formation of Neisseria meningitidis}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20180907}, volume = 75, year = 2010 }