Invertase was covalently immobilized on glass-ceramic support (G(S)
obtained from coal fly ashes (CFA) with polyvinyl pyrrolidone (PVP) and
magnesium stearate added as temporary additive. The GCS samples
containing Pb(CH3COO)(2), (GCSPb) or ZnSO4 (GCSZn) were obtained in
several sintering temperatures (1000-1200 degrees C) and times (1-3 h).
The apparent values of porosity, water absorption, density and uniaxial
compressive strength were studied by factorial design and morphological
differences by scanning electron microscope. The best covalent
immobilized invertase derivative was on GCSZn (1200 degrees C/1 h) with
4398 +/- 59.75 U/g GCS. However, 95.83% of immobilized invertase
activity was obtained using an economical protocol with concentration of
0.636 mM 3-aminopropyltriethoxy silane (3-APTES) and 0.576 mM
glutaraldehycle. The GCSZn-invertase derivative retained 100% of
initial activity after nine reuses (5476.19 +/- 155.49 U/g GCSZn). The
CFA showed to be a promising biotechnological alternative as
immobilization support for production of invert sugar and may be for
other industrial enzymes. (C) 2012 Elsevier B.V. All rights reserved.
%0 Journal Article
%1 WOS:000302824400040
%A Albertini, A V P
%A Cadena, P G
%A Silva, J L
%A Nascimento, G A
%A Reis, A L S
%A Freire, V N
%A Santos, R P
%A Martins, J L
%A Cavada, B S
%A Neto, P J Rolim
%A Pimentel, M C B
%A Martinez, C R
%A Porto, A L F
%A Filho, J L Lima
%C PO BOX 564, 1001 LAUSANNE, SWITZERLAND
%D 2012
%I ELSEVIER SCIENCE SA
%J CHEMICAL ENGINEERING JOURNAL
%K Glass-ceramic Immobilization; Invertase; Performance} ashes; fly supports; {Coal
%P 341-350
%R 10.1016/j.cej.2012.01.091
%T Performance of invertase immobilized on glass-ceramic supports in batch
bioreactor
%V 187
%X Invertase was covalently immobilized on glass-ceramic support (G(S)
obtained from coal fly ashes (CFA) with polyvinyl pyrrolidone (PVP) and
magnesium stearate added as temporary additive. The GCS samples
containing Pb(CH3COO)(2), (GCSPb) or ZnSO4 (GCSZn) were obtained in
several sintering temperatures (1000-1200 degrees C) and times (1-3 h).
The apparent values of porosity, water absorption, density and uniaxial
compressive strength were studied by factorial design and morphological
differences by scanning electron microscope. The best covalent
immobilized invertase derivative was on GCSZn (1200 degrees C/1 h) with
4398 +/- 59.75 U/g GCS. However, 95.83% of immobilized invertase
activity was obtained using an economical protocol with concentration of
0.636 mM 3-aminopropyltriethoxy silane (3-APTES) and 0.576 mM
glutaraldehycle. The GCSZn-invertase derivative retained 100% of
initial activity after nine reuses (5476.19 +/- 155.49 U/g GCSZn). The
CFA showed to be a promising biotechnological alternative as
immobilization support for production of invert sugar and may be for
other industrial enzymes. (C) 2012 Elsevier B.V. All rights reserved.
@article{WOS:000302824400040,
abstract = {Invertase was covalently immobilized on glass-ceramic support (G(S)
obtained from coal fly ashes (CFA) with polyvinyl pyrrolidone (PVP) and
magnesium stearate added as temporary additive. The GCS samples
containing Pb(CH3COO)(2), (GCSPb) or ZnSO4 (GCSZn) were obtained in
several sintering temperatures (1000-1200 degrees C) and times (1-3 h).
The apparent values of porosity, water absorption, density and uniaxial
compressive strength were studied by factorial design and morphological
differences by scanning electron microscope. The best covalent
immobilized invertase derivative was on GCSZn (1200 degrees C/1 h) with
4398 +/- 59.75 U/g GCS. However, 95.83% of immobilized invertase
activity was obtained using an economical protocol with concentration of
0.636 mM 3-aminopropyltriethoxy silane (3-APTES) and 0.576 mM
glutaraldehycle. The GCSZn-invertase derivative retained 100% of
initial activity after nine reuses (5476.19 +/- 155.49 U/g GCSZn). The
CFA showed to be a promising biotechnological alternative as
immobilization support for production of invert sugar and may be for
other industrial enzymes. (C) 2012 Elsevier B.V. All rights reserved.},
added-at = {2022-05-23T20:00:14.000+0200},
address = {PO BOX 564, 1001 LAUSANNE, SWITZERLAND},
author = {Albertini, A V P and Cadena, P G and Silva, J L and Nascimento, G A and Reis, A L S and Freire, V N and Santos, R P and Martins, J L and Cavada, B S and Neto, P J Rolim and Pimentel, M C B and Martinez, C R and Porto, A L F and Filho, J L Lima},
biburl = {https://www.bibsonomy.org/bibtex/2b223da636be280e94fbf8fcca02bb60b/ppgfis_ufc_br},
doi = {10.1016/j.cej.2012.01.091},
interhash = {962d8f84ae46840a9f585f477903625b},
intrahash = {b223da636be280e94fbf8fcca02bb60b},
issn = {1385-8947},
journal = {CHEMICAL ENGINEERING JOURNAL},
keywords = {Glass-ceramic Immobilization; Invertase; Performance} ashes; fly supports; {Coal},
pages = {341-350},
publisher = {ELSEVIER SCIENCE SA},
pubstate = {published},
timestamp = {2022-05-23T20:00:14.000+0200},
title = {Performance of invertase immobilized on glass-ceramic supports in batch
bioreactor},
tppubtype = {article},
volume = 187,
year = 2012
}