The ability of aerobic anoxygenic photoheterotrophs (AAPs) to gain additional energy from sunlight represents a competitive advantage, especially in conditions where light has easy access or under environmental conditions may change quickly, such as those in the world´s oceans. However, the knowledge about the metabolic consequences of aerobic anoxygenic photosynthesis is very limited. Combining transcriptome and metabolome analyses, isotopic labelling techniques, measurements of growth, oxygen uptake rates, flow cytometry, and a number of other biochemical analytical techniques we obtained a comprehensive overview on the complex adaption of the marine bacterium Dinoroseobacter shibae DFL12T during transition from heterotrophy to photoheterotrophy (growth on succinate). Growth in light was characterized by reduced respiration, a decreased metabolic flux through the tricarboxylic acid (TCA) cycle and the assimilation of CO2 via an enhanced flux through the ethylmalonyl-CoA (EMC) pathway, which was shown to be connected to the serine metabolism. Adaptation to photoheterotrophy is mainly characterized by metabolic reactions caused by a surplus of reducing potential and might depend on genes located in one operon, encoding branching point enzymes of the EMC pathway, serine metabolism and the TCA cycle.
%0 Journal Article
%1 EMI:EMI13746
%A Bill, Nelli
%A Tomasch, Jürgen
%A Riemer, Alexander
%A Müller, Katrin
%A Kleist, Sarah
%A Schmidt-Hohagen, Kerstin
%A Wagner-Döbler, Irene
%A Schomburg, Dietmar
%D 2017
%J Environmental Microbiology
%K Dinoroseobacter ethylmalonyl-CoA myown pathway shibae
%N 7
%P 2645--2660
%R 10.1111/1462-2920.13746
%T Fixation of CO2 using the ethylmalonyl-CoA pathway in the photoheterotrophic marine bacterium Dinoroseobacter shibae
%U http://dx.doi.org/10.1111/1462-2920.13746
%V 19
%X The ability of aerobic anoxygenic photoheterotrophs (AAPs) to gain additional energy from sunlight represents a competitive advantage, especially in conditions where light has easy access or under environmental conditions may change quickly, such as those in the world´s oceans. However, the knowledge about the metabolic consequences of aerobic anoxygenic photosynthesis is very limited. Combining transcriptome and metabolome analyses, isotopic labelling techniques, measurements of growth, oxygen uptake rates, flow cytometry, and a number of other biochemical analytical techniques we obtained a comprehensive overview on the complex adaption of the marine bacterium Dinoroseobacter shibae DFL12T during transition from heterotrophy to photoheterotrophy (growth on succinate). Growth in light was characterized by reduced respiration, a decreased metabolic flux through the tricarboxylic acid (TCA) cycle and the assimilation of CO2 via an enhanced flux through the ethylmalonyl-CoA (EMC) pathway, which was shown to be connected to the serine metabolism. Adaptation to photoheterotrophy is mainly characterized by metabolic reactions caused by a surplus of reducing potential and might depend on genes located in one operon, encoding branching point enzymes of the EMC pathway, serine metabolism and the TCA cycle.
@article{EMI:EMI13746,
abstract = {The ability of aerobic anoxygenic photoheterotrophs (AAPs) to gain additional energy from sunlight represents a competitive advantage, especially in conditions where light has easy access or under environmental conditions may change quickly, such as those in the world´s oceans. However, the knowledge about the metabolic consequences of aerobic anoxygenic photosynthesis is very limited. Combining transcriptome and metabolome analyses, isotopic labelling techniques, measurements of growth, oxygen uptake rates, flow cytometry, and a number of other biochemical analytical techniques we obtained a comprehensive overview on the complex adaption of the marine bacterium Dinoroseobacter shibae DFL12T during transition from heterotrophy to photoheterotrophy (growth on succinate). Growth in light was characterized by reduced respiration, a decreased metabolic flux through the tricarboxylic acid (TCA) cycle and the assimilation of CO2 via an enhanced flux through the ethylmalonyl-CoA (EMC) pathway, which was shown to be connected to the serine metabolism. Adaptation to photoheterotrophy is mainly characterized by metabolic reactions caused by a surplus of reducing potential and might depend on genes located in one operon, encoding branching point enzymes of the EMC pathway, serine metabolism and the TCA cycle.},
added-at = {2017-07-27T09:24:54.000+0200},
author = {Bill, Nelli and Tomasch, Jürgen and Riemer, Alexander and Müller, Katrin and Kleist, Sarah and Schmidt-Hohagen, Kerstin and Wagner-Döbler, Irene and Schomburg, Dietmar},
biburl = {https://www.bibsonomy.org/bibtex/2dedb9eb0c9fcd6e11ee1a7bd99f57bd0/brenda_bs},
doi = {10.1111/1462-2920.13746},
interhash = {fcda17c58269e130e1110f8247e345aa},
intrahash = {dedb9eb0c9fcd6e11ee1a7bd99f57bd0},
issn = {1462-2920},
journal = {Environmental Microbiology},
keywords = {Dinoroseobacter ethylmalonyl-CoA myown pathway shibae},
number = 7,
pages = {2645--2660},
timestamp = {2017-10-30T09:32:00.000+0100},
title = {Fixation of CO2 using the ethylmalonyl-CoA pathway in the photoheterotrophic marine bacterium Dinoroseobacter shibae},
url = {http://dx.doi.org/10.1111/1462-2920.13746},
volume = 19,
year = 2017
}