%0 Journal Article %1 RN231 %A Toral, M.I. %A Nacaratte, F. %A Nova, F. %D 2015 %J Analytical Letters %K contraceptive cyproterone-acetate, desogestrel, dqcauchile estradiol, ethinyl etonogestrel, formulation, intrauterine mass-spectrometry, pharmaceutical plasma properties, release ring, spectrophotometry, %N 6 %P 1009-1020 %R 10.1080/00032719.2014.968930 %T Determination of Etonogestrel and Ethinyl Estradiol from an Intrauterine Contraceptive Ring by Extraction and Derivative Spectrophotometry %U /brokenurl#<Go to ISI>://WOS:000348519800011 %V 48 %X A new method for the determination and extraction of ethinyl estradiol and etonogestrel was developed for a pharmaceutical product consisting of a solid copolymer intrauterine ring for subsequent simultaneous quantification by ultraviolet-visible derivative spectrophotometry. The spectral variables were optimized for first derivative spectrophotometry with a smoothing factor of 4000 and an amplification factor of 10,000. A wavelength of 291.5nm was selected for the determination of ethinyl estradiol by a graphical method, while for etonogestrel, zero-crossing was used at 249.5nm. The limits of detection and quantification for ethinyl estradiol were 9.5x10(-7) and 9.0x10(-6)mol/L and 1.1x10(-6) and 3.2x10(-6)mol/L for etonogestrel, respectively. For the extraction method, the variables affecting the analytical signal were the extraction solvent, temperature, and extraction time; optimized conditions were 180min at 76 +/- 2 degrees C in acetonitrile. The method was applied successfully for the first time to analyze intrauterine contraceptive rings. The sample contained 11.7mg etonogestrel and 2.7mg ethinyl estradiol; the recoveries were 93.6 +/- 1.1% etonogestrel and 97.9 +/- 1.5% ethinyl estradiol relative to the nominal concentration. The importance of this method involves its implementation in pharmaceutical laboratories.

@article{RN231, abstract = {A new method for the determination and extraction of ethinyl estradiol and etonogestrel was developed for a pharmaceutical product consisting of a solid copolymer intrauterine ring for subsequent simultaneous quantification by ultraviolet-visible derivative spectrophotometry. The spectral variables were optimized for first derivative spectrophotometry with a smoothing factor of 4000 and an amplification factor of 10,000. A wavelength of 291.5nm was selected for the determination of ethinyl estradiol by a graphical method, while for etonogestrel, zero-crossing was used at 249.5nm. The limits of detection and quantification for ethinyl estradiol were 9.5x10(-7) and 9.0x10(-6)mol/L and 1.1x10(-6) and 3.2x10(-6)mol/L for etonogestrel, respectively. For the extraction method, the variables affecting the analytical signal were the extraction solvent, temperature, and extraction time; optimized conditions were 180min at 76 +/- 2 degrees C in acetonitrile. The method was applied successfully for the first time to analyze intrauterine contraceptive rings. The sample contained 11.7mg etonogestrel and 2.7mg ethinyl estradiol; the recoveries were 93.6 +/- 1.1% etonogestrel and 97.9 +/- 1.5% ethinyl estradiol relative to the nominal concentration. The importance of this method involves its implementation in pharmaceutical laboratories.}, added-at = {2019-12-04T03:57:35.000+0100}, author = {Toral, M.I. and Nacaratte, F. and Nova, F.}, biburl = {https://www.bibsonomy.org/bibtex/2b6067abaf9bea1dedff9529041898faa/dqcauchile}, doi = {10.1080/00032719.2014.968930}, interhash = {6b6bd524e5c05400a5d9ec0d2c530ec2}, intrahash = {b6067abaf9bea1dedff9529041898faa}, issn = {0003-2719}, journal = {Analytical Letters}, keywords = {contraceptive cyproterone-acetate, desogestrel, dqcauchile estradiol, ethinyl etonogestrel, formulation, intrauterine mass-spectrometry, pharmaceutical plasma properties, release ring, spectrophotometry,}, number = 6, pages = {1009-1020}, timestamp = {2019-12-04T03:58:17.000+0100}, title = {Determination of Etonogestrel and Ethinyl Estradiol from an Intrauterine Contraceptive Ring by Extraction and Derivative Spectrophotometry}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000348519800011}, volume = 48, year = 2015 }