%0 Journal Article %1 WOS:000412676800030 %A Vieira-Damiani, Gislaine %A Lage, Denise %A Daldon, Patricia Erica Christofoletti %A Alves, Caroline Romanelli Tiburcio %A Cintra, Maria Leticia %A Metze, Konradin %A Adur, Javier %A Pelegati, Vitor B %A Carvalho, Hernandes F %A Cesar, Carlos Lenz %C 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA %D 2017 %I MOSBY-ELSEVIER %J JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY %K 2-photon Pasini Pierini; SHG; TPEF; and atrophoderma atrophy; collagen elastic excited fibers; fluorescence} generation; harmonic idiopathic morphometry; of pathology; scleroderma; second {atrophoderma; %N 5 %P 930-937 %R 10.1016/j.jaad.2017.02.044 %T Idiopathic atrophoderma of Pasini and Pierini: A case study of collagen and elastin texture by multiphoton microscopy %V 77 %X Background: The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. Objective: To identify histologic features that distinguish IAPP from unaffected skin. Methods: Eleven patients were included for conventional analyses. Masson trichromee and Unna-Tanzer orcein-stained sections were evaluated using automated morphometry. Hematoxylin-eosinestained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation. Results: No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis. Limitations: The low number of patients evaluated. Conclusion: The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses.

@article{WOS:000412676800030, abstract = {Background: The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. Objective: To identify histologic features that distinguish IAPP from unaffected skin. Methods: Eleven patients were included for conventional analyses. Masson trichromee and Unna-Tanzer orcein-stained sections were evaluated using automated morphometry. Hematoxylin-eosinestained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation. Results: No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis. Limitations: The low number of patients evaluated. Conclusion: The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA}, author = {Vieira-Damiani, Gislaine and Lage, Denise and Daldon, Patricia Erica Christofoletti and Alves, Caroline Romanelli Tiburcio and Cintra, Maria Leticia and Metze, Konradin and Adur, Javier and Pelegati, Vitor B and Carvalho, Hernandes F and Cesar, Carlos Lenz}, biburl = {https://www.bibsonomy.org/bibtex/2cb18982b2d23954ede1e0c8468b18391/ppgfis_ufc_br}, doi = {10.1016/j.jaad.2017.02.044}, interhash = {7baccb89fb2763e8eece09ce89648897}, intrahash = {cb18982b2d23954ede1e0c8468b18391}, issn = {0190-9622}, journal = {JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY}, keywords = {2-photon Pasini Pierini; SHG; TPEF; and atrophoderma atrophy; collagen elastic excited fibers; fluorescence} generation; harmonic idiopathic morphometry; of pathology; scleroderma; second {atrophoderma;}, number = 5, pages = {930-937}, publisher = {MOSBY-ELSEVIER}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Idiopathic atrophoderma of Pasini and Pierini: A case study of collagen and elastin texture by multiphoton microscopy}, tppubtype = {article}, volume = 77, year = 2017 }