%0 Journal Article %1 christian2011small %A Christian, F. %A Szaszák, M. %A Friedl, S. %A Drewianka, S. %A Lorenz, D. %A Goncalves, A. %A Furkert, J. %A Vargas, C. %A Schmieder, P. %A Götz, F. %A Zühlke, K. %A Moutty, M. %A Göttert, H. %A Joshi, M. %A Reif, B. %A Haase, H. %A Morano, I. %A Grossmann, S. %A Klukovits, A. %A Verli, J. %A Gáspár, R. %A Noack, C. %A Bergmann, M. %A Kass, R. %A Hampel, K. %A Kashin, D. %A Genieser, H. G. %A Herberg, F. W. %A Willoughby, D. %A Cooper, D. M. %A Baillie, G. S. %A Houslay, M. D. %A Kries, J. P. von %A Zimmermann, B. %A Rosenthal, W. %A Klussmann, E. %D 2011 %J J Biol Chem %K herberg myown %N 11 %P 9079-9096 %R 10.1074/jbc.M110.160614 %T Small molecule AKAP-protein kinase A (PKA) interaction disruptors that activate PKA interfere with compartmentalized cAMP signaling in cardiac myocytes %U http://www.ncbi.nlm.nih.gov/pubmed/21177871?dopt=Abstract %V 286 %X A-kinase anchoring proteins (AKAPs) tether protein kinase A (PKA) and other signaling proteins to defined intracellular sites, thereby establishing compartmentalized cAMP signaling. AKAP-PKA interactions play key roles in various cellular processes, including the regulation of cardiac myocyte contractility. We discovered small molecules, 3,3'-diamino-4,4'-dihydroxydiphenylmethane (FMP-API-1) and its derivatives, which inhibit AKAP-PKA interactions in vitro and in cultured cardiac myocytes. The molecules bind to an allosteric site of regulatory subunits of PKA identifying a hitherto unrecognized region that controls AKAP-PKA interactions. FMP-API-1 also activates PKA. The net effect of FMP-API-1 is a selective interference with compartmentalized cAMP signaling. In cardiac myocytes, FMP-API-1 reveals a novel mechanism involved in terminating β-adrenoreceptor-induced cAMP synthesis. In addition, FMP-API-1 leads to an increase in contractility of cultured rat cardiac myocytes and intact hearts. Thus, FMP-API-1 represents not only a novel means to study compartmentalized cAMP/PKA signaling but, due to its effects on cardiac myocytes and intact hearts, provides the basis for a new concept in the treatment of chronic heart failure.

@article{christian2011small, abstract = {A-kinase anchoring proteins (AKAPs) tether protein kinase A (PKA) and other signaling proteins to defined intracellular sites, thereby establishing compartmentalized cAMP signaling. AKAP-PKA interactions play key roles in various cellular processes, including the regulation of cardiac myocyte contractility. We discovered small molecules, 3,3'-diamino-4,4'-dihydroxydiphenylmethane (FMP-API-1) and its derivatives, which inhibit AKAP-PKA interactions in vitro and in cultured cardiac myocytes. The molecules bind to an allosteric site of regulatory subunits of PKA identifying a hitherto unrecognized region that controls AKAP-PKA interactions. FMP-API-1 also activates PKA. The net effect of FMP-API-1 is a selective interference with compartmentalized cAMP signaling. In cardiac myocytes, FMP-API-1 reveals a novel mechanism involved in terminating β-adrenoreceptor-induced cAMP synthesis. In addition, FMP-API-1 leads to an increase in contractility of cultured rat cardiac myocytes and intact hearts. Thus, FMP-API-1 represents not only a novel means to study compartmentalized cAMP/PKA signaling but, due to its effects on cardiac myocytes and intact hearts, provides the basis for a new concept in the treatment of chronic heart failure.}, added-at = {2011-10-24T13:52:24.000+0200}, author = {Christian, F. and Szaszák, M. and Friedl, S. and Drewianka, S. and Lorenz, D. and Goncalves, A. and Furkert, J. and Vargas, C. and Schmieder, P. and Götz, F. and Zühlke, K. and Moutty, M. and Göttert, H. and Joshi, M. and Reif, B. and Haase, H. and Morano, I. and Grossmann, S. and Klukovits, A. and Verli, J. and Gáspár, R. and Noack, C. and Bergmann, M. and Kass, R. and Hampel, K. and Kashin, D. and Genieser, H. G. and Herberg, F. W. and Willoughby, D. and Cooper, D. M. and Baillie, G. S. and Houslay, M. D. and Kries, J. P. von and Zimmermann, B. and Rosenthal, W. and Klussmann, E.}, biburl = {https://www.bibsonomy.org/bibtex/222fd3df75429ead5ccf5852a9ddf2a66/biochemie}, doi = {10.1074/jbc.M110.160614}, interhash = {826152c3b44eb65ecd70c70883320827}, intrahash = {22fd3df75429ead5ccf5852a9ddf2a66}, journal = {J Biol Chem}, keywords = {herberg myown}, month = mar, number = 11, pages = {9079-9096}, pmid = {21177871}, timestamp = {2012-07-24T09:38:39.000+0200}, title = {Small molecule AKAP-protein kinase A (PKA) interaction disruptors that activate PKA interfere with compartmentalized cAMP signaling in cardiac myocytes}, url = {http://www.ncbi.nlm.nih.gov/pubmed/21177871?dopt=Abstract}, volume = 286, year = 2011 }