%0 Journal Article %1 citeulike:566864 %A Marcovina, S. M. %A Adolphson, J. L. %A Parlavecchia, M. %A Albers, J. J. %C Northwest Lipid Research Center, Harborview Medical Center, Seattle, WA 98104-2499. %D 1990 %J Clin Chem %K methodology immunoassay apob %N 2 %P 366--369 %T Effects of lyophilization of serum on the measurement of apolipoproteins A-I and B. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2105863 %V 36 %X A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B. We determined values for apolipoproteins A-I and B in frozen and lyophilized serum pools, using different immunochemical approaches. We found little or no differences in apolipoprotein A-I values between frozen and lyophilized pools as determined by the different methods. In contrast, values for apolipoprotein B in lyophilized samples were consistently lower than those obtained for frozen samples. After adjusting for the effect of dilution due to reconstitution, the difference in the apolipoprotein B values for lyophilized as compared with frozen samples ranged from -26% to 4%, depending upon the assay method. Evidently, serum pools in lyophilized from are not a suitable matrix for reference materials for apolipoprotein B measurements but can be used for apolipoprotein A-I measurements.

@article{citeulike:566864, abstract = {A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B. We determined values for apolipoproteins A-I and B in frozen and lyophilized serum pools, using different immunochemical approaches. We found little or no differences in apolipoprotein A-I values between frozen and lyophilized pools as determined by the different methods. In contrast, values for apolipoprotein B in lyophilized samples were consistently lower than those obtained for frozen samples. After adjusting for the effect of dilution due to reconstitution, the difference in the apolipoprotein B values for lyophilized as compared with frozen samples ranged from -26% to 4%, depending upon the assay method. Evidently, serum pools in lyophilized from are not a suitable matrix for reference materials for apolipoprotein B measurements but can be used for apolipoprotein A-I measurements.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Northwest Lipid Research Center, Harborview Medical Center, Seattle, WA 98104-2499.}, author = {Marcovina, S. M. and Adolphson, J. L. and Parlavecchia, M. and Albers, J. J.}, biburl = {https://www.bibsonomy.org/bibtex/22c5b4cd125fe6db9d53102db776d8543/biblio24}, citeulike-article-id = {566864}, interhash = {98418f85bf365c3a458c9fa7ce2a46ae}, intrahash = {2c5b4cd125fe6db9d53102db776d8543}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {methodology immunoassay apob}, month = {February}, number = 2, pages = {366--369}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Effects of lyophilization of serum on the measurement of apolipoproteins A-I and B.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=2105863}, volume = 36, year = 1990 }