%0 Journal Article %1 Jaeger1990 %A Jaeger, C. B. %A Greene, L. A. %A Tresco, P. A. %A Winn, S. R. %A Aebischer, P. %D 1990 %J Prog Brain Res %K *Brain *Prostheses *Transplantation, ; Adrenal Animals Artificial Cell Cells, Compounding Cultured/*transplantation Disease/*surgery Division Dopamine/*secretion Drug Gland Gov't, Graft Heterotopic Implants Line Membranes, Mice Neoplasms/pathology Neuroblastoma/pathology P.H.S. Parkinson Pheochromocytoma/pathology Rats Research Support, Survival Tumor U.S. and %P 41-6 %T Polymer encapsulated dopaminergic cell lines as älternative neural grafts". %U http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?cmd=prlinks&dbfrom=pubmed&retmode=ref&id=2290954 %V 82 %X Our preliminary findings (Jaeger et al., 1988; Aebischer et al., 1989; Tresco et al., 1989) and the studies in progress show that encapsulated dopaminergic cell lines survive enclosure within a semi-permeable membrane. The encapsulated cells remained viable for extended time periods when maintained in vitro. Moreover, encapsulated PC12 and T28 cells have the potential to survive following their implantation into the forebrain of rats. Cell lines are essentially "immortal" because they continue to divide indefinitely. This property allows perpetual "self-renewal" of a given cell population. However, the capacity of continuous uncontrolled cell division may also lead to tumor formation. This in fact is the case for unencapsulated PC12 cell implants placed into the brain of young Sprague Dawley rats (Jaeger, 1985). Cell line encapsulation has the potential to prevent tumor growth (Jaeger et al., 1988). Survival for 6 months in vitro suggests that encapsulation does not preclude long-term maintenance of an homogeneous cell line like PC12 cells. The presence of mitotic figures in the capsules further supports the likelihood of propagation and self renewal of the encapsulated population. Another significant property of cell lines is that they consist of a single, genetically homogeneous cell type. They do not require specific synaptic interactions for their survival. In the case of PC12 and T28 lines, the cells synthesize and release neurotransmitters. Our data show that PC12 and T28 cells continue to release dopamine spontaneously and to express specific transmitters and enzymes following encapsulation. Thus, cell lines such as these may constitute relatively simple "neural implants" exerting their function via humoral release.(ABSTRACT TRUNCATED AT 250 WORDS)

@article{Jaeger1990, __markedentry = {[phpts:6]}, abstract = {Our preliminary findings (Jaeger et al., 1988; Aebischer et al., 1989; Tresco et al., 1989) and the studies in progress show that encapsulated dopaminergic cell lines survive enclosure within a semi-permeable membrane. The encapsulated cells remained viable for extended time periods when maintained in vitro. Moreover, encapsulated PC12 and T28 cells have the potential to survive following their implantation into the forebrain of rats. Cell lines are essentially "immortal" because they continue to divide indefinitely. This property allows perpetual "self-renewal" of a given cell population. However, the capacity of continuous uncontrolled cell division may also lead to tumor formation. This in fact is the case for unencapsulated PC12 cell implants placed into the brain of young Sprague Dawley rats (Jaeger, 1985). Cell line encapsulation has the potential to prevent tumor growth (Jaeger et al., 1988). Survival for 6 months in vitro suggests that encapsulation does not preclude long-term maintenance of an homogeneous cell line like PC12 cells. The presence of mitotic figures in the capsules further supports the likelihood of propagation and self renewal of the encapsulated population. Another significant property of cell lines is that they consist of a single, genetically homogeneous cell type. They do not require specific synaptic interactions for their survival. In the case of PC12 and T28 lines, the cells synthesize and release neurotransmitters. Our data show that PC12 and T28 cells continue to release dopamine spontaneously and to express specific transmitters and enzymes following encapsulation. Thus, cell lines such as these may constitute relatively simple "neural implants" exerting their function via humoral release.(ABSTRACT TRUNCATED AT 250 WORDS)}, added-at = {2011-11-04T13:47:04.000+0100}, author = {Jaeger, C. B. and Greene, L. A. and Tresco, P. A. and Winn, S. R. and Aebischer, P.}, authoraddress = {Department of Anatomy, Purdue University, School of Veterinary Medicine, West Lafayette, IN 47907.}, biburl = {https://www.bibsonomy.org/bibtex/2b95ac6276e0d7494fdaa1ef67f8cef56/pawelsikorski}, interhash = {a88d684d22114d98483507b22c6afbe2}, intrahash = {b95ac6276e0d7494fdaa1ef67f8cef56}, journal = {Prog Brain Res}, keywords = {*Brain *Prostheses *Transplantation, ; Adrenal Animals Artificial Cell Cells, Compounding Cultured/*transplantation Disease/*surgery Division Dopamine/*secretion Drug Gland Gov't, Graft Heterotopic Implants Line Membranes, Mice Neoplasms/pathology Neuroblastoma/pathology P.H.S. Parkinson Pheochromocytoma/pathology Rats Research Support, Survival Tumor U.S. and}, language = {eng}, medline-da = {19910404}, medline-dcom = {19910404}, medline-edat = {1990/01/01}, medline-fau = {Jaeger, C B ; Greene, L A ; Tresco, P A ; Winn, S R ; Aebischer, P}, medline-gr = {NS 27694/NS/NINDS}, medline-is = {0079-6123 (Print)}, medline-jid = {0376441}, medline-jt = {Progress in brain research.}, medline-lr = {20060417}, medline-mhda = {1990/01/01 00:01}, medline-own = {NLM}, medline-pl = {NETHERLANDS}, medline-pmid = {2290954}, medline-pst = {ppublish}, medline-pt = {Journal Article}, medline-pubm = {Print}, medline-rn = {0 (Membranes, Artificial) ; 51-61-6 (Dopamine)}, medline-sb = {IM}, medline-so = {Prog Brain Res. 1990;82:41-6.}, medline-stat = {MEDLINE}, owner = {phpts}, pages = {41-6}, timestamp = {2011-11-04T13:47:13.000+0100}, title = {Polymer encapsulated dopaminergic cell lines as "alternative neural grafts".}, url = {http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?cmd=prlinks\&dbfrom=pubmed\&retmode=ref\&id=2290954}, volume = 82, year = 1990 }