Changes in measured image irradiance have many physical causes and are the primary cue for several visual processes, such as edge detection and shape from shading. Using physical models for charged-coupled device (CCD) video cameras and material reflectance, we quantify the variation in digitized pixel values that is due to sensor noise and scene variation. This analysis forms the basis of algorithms for camera characterization and calibration and for scene description. Specifically, algorithms are developed for estimating the parameters of camera noise and for calibrating a camera to remove the effects of fixed pattern nonuniformity and spatial variation in dark current. While these techniques have many potential uses, we describe in particular how they can be used to estimate a measure of scene variation. This measure is independent of image irradiance and can be used to identify a surface from a single sensor band over a range of situations.