Infectious hepatitis B virus (HBV), namely Dane particles (DPs), consists of a core nucleocapsid including genome DNA covered with an envelope of hepatitis B surface antigen (HBsAg). We report the synthesis, structure, and HBV-trapping capability of multilayered protein nanotubes having an anti-HBsAg antibody (HBsAb) layer as an internal wall. The nanotubes were prepared using an alternating layer-by-layer assembly of human serum albumin (HSA) and oppositely charged poly-l-arginine (PLA) into a nanoporous polycarbonate (PC) membrane (pore size, 400 nm), followed by depositions of poly-l-glutamic acid (PLG) and HBsAb. Subsequent dissolution of the PC template yielded (PLA/HSA)2PLA/PLG/HBsAb nanotubes (AbNTs). The SEM measurements revealed the formation of uniform hollow cylinders with a 414 ± 16 nm outer diameter and 59 ± 4 nm wall thickness. In an aqueous medium, the swelled nanotubes captured noninfectious spherical small particles of HBsAg (SPs); the binding constant was 3.5 à 107 Mâ1. Surprisingly, the amount of genome DNA in the HBV solution (HBsAg-positive plasma or DP-rich solution) decreased dramatically after incubation with the AbNTs (â3.9âlog order), which implies that the infectious DPs were completely entrapped into the one-dimensional pore space of the AbNTs.
Beschreibung
Virus Trap in Human Serum Albumin Nanotube - Journal of the American Chemical Society (ACS Publications)
%0 Journal Article
%1 komatsu2011virus
%A Komatsu, Teruyuki
%A Qu, Xue
%A Ihara, Hiromi
%A Fujihara, Mitsuhiro
%A Azuma, Hiroshi
%A Ikeda, Hisami
%D 2011
%J Journal of the American Chemical Society
%K albumin lbl nanotubes virus
%N 10
%P 3246-3248
%R 10.1021/ja1096122
%T Virus Trap in Human Serum Albumin Nanotube
%U http://pubs.acs.org/doi/abs/10.1021/ja1096122
%V 133
%X Infectious hepatitis B virus (HBV), namely Dane particles (DPs), consists of a core nucleocapsid including genome DNA covered with an envelope of hepatitis B surface antigen (HBsAg). We report the synthesis, structure, and HBV-trapping capability of multilayered protein nanotubes having an anti-HBsAg antibody (HBsAb) layer as an internal wall. The nanotubes were prepared using an alternating layer-by-layer assembly of human serum albumin (HSA) and oppositely charged poly-l-arginine (PLA) into a nanoporous polycarbonate (PC) membrane (pore size, 400 nm), followed by depositions of poly-l-glutamic acid (PLG) and HBsAb. Subsequent dissolution of the PC template yielded (PLA/HSA)2PLA/PLG/HBsAb nanotubes (AbNTs). The SEM measurements revealed the formation of uniform hollow cylinders with a 414 ± 16 nm outer diameter and 59 ± 4 nm wall thickness. In an aqueous medium, the swelled nanotubes captured noninfectious spherical small particles of HBsAg (SPs); the binding constant was 3.5 à 107 Mâ1. Surprisingly, the amount of genome DNA in the HBV solution (HBsAg-positive plasma or DP-rich solution) decreased dramatically after incubation with the AbNTs (â3.9âlog order), which implies that the infectious DPs were completely entrapped into the one-dimensional pore space of the AbNTs.
@article{komatsu2011virus,
abstract = { Infectious hepatitis B virus (HBV), namely Dane particles (DPs), consists of a core nucleocapsid including genome DNA covered with an envelope of hepatitis B surface antigen (HBsAg). We report the synthesis, structure, and HBV-trapping capability of multilayered protein nanotubes having an anti-HBsAg antibody (HBsAb) layer as an internal wall. The nanotubes were prepared using an alternating layer-by-layer assembly of human serum albumin (HSA) and oppositely charged poly-l-arginine (PLA) into a nanoporous polycarbonate (PC) membrane (pore size, 400 nm), followed by depositions of poly-l-glutamic acid (PLG) and HBsAb. Subsequent dissolution of the PC template yielded (PLA/HSA)2PLA/PLG/HBsAb nanotubes (AbNTs). The SEM measurements revealed the formation of uniform hollow cylinders with a 414 ± 16 nm outer diameter and 59 ± 4 nm wall thickness. In an aqueous medium, the swelled nanotubes captured noninfectious spherical small particles of HBsAg (SPs); the binding constant was 3.5 à 107 Mâ1. Surprisingly, the amount of genome DNA in the HBV solution (HBsAg-positive plasma or DP-rich solution) decreased dramatically after incubation with the AbNTs (â3.9âlog order), which implies that the infectious DPs were completely entrapped into the one-dimensional pore space of the AbNTs. },
added-at = {2011-05-31T08:24:14.000+0200},
author = {Komatsu, Teruyuki and Qu, Xue and Ihara, Hiromi and Fujihara, Mitsuhiro and Azuma, Hiroshi and Ikeda, Hisami},
biburl = {https://www.bibsonomy.org/bibtex/2acb2c6b44931cb5ae7818f466969bf87/saghi},
description = {Virus Trap in Human Serum Albumin Nanotube - Journal of the American Chemical Society (ACS Publications)},
doi = {10.1021/ja1096122},
eprint = {http://pubs.acs.org/doi/pdf/10.1021/ja1096122},
interhash = {5d0c717912875fbfc5f0f947f093e1a6},
intrahash = {acb2c6b44931cb5ae7818f466969bf87},
journal = {Journal of the American Chemical Society},
keywords = {albumin lbl nanotubes virus},
number = 10,
pages = {3246-3248},
timestamp = {2011-05-31T08:24:14.000+0200},
title = {Virus Trap in Human Serum Albumin Nanotube},
url = {http://pubs.acs.org/doi/abs/10.1021/ja1096122},
volume = 133,
year = 2011
}